Seed sources, seedling culture, and common garden design
Seeds were collected from 30 maternal trees representing 10 populations
(3 maternal trees/population; Fig. 1b) occurring across the Pinus
strobiformis - P. flexilis hybrid zone in Arizona, Colorado, New
Mexico, and Texas (Menon et al ., 2018). Cones were dried and
processed prior to seed sowing and the first growth season in the
Research Greenhouse at Northern Arizona University as described in
Bucholz et al . (2020). Replicates of each maternal family in two
gardens were planted into 107 cm × 117 cm × 91 cm raised bed garden
boxes constructed at two Southwest Experimental Garden Array (SEGA)
common gardens in the Kaibab National Forest, AZ (Fig. 1) also as
described in Bucholz et al . (2020). Sets of seedlings from one
tree are mixed-sib families (maternal families), and it is unknown
whether seedlings within a maternal family are full or half siblings.
Further details about seed sources and common garden design are detailed
in Bucholz et al . (2020).