Fig. 6 Schematic representation of the synergistic antimicrobial mechanism of R12-AgNPs with H2O2.
Biocompatibility evaluation of R12-AgNPs
The biocompatibility of nanoparticles is a critical factor in determining their potential biomedical applications. Therefore, it is essential to evaluate the biocompatibility of R12-AgNPs. The cytotoxicity of R12-AgNPs was assessed in vitro using mouse fibroblast (L929). As shown in Fig. 7, the cell viability remained at 92.39% when the concentration of R12-AgNPs was below 10 µg/mL. Even a higher concentration of 75 µg/mL, a cell viability of 80% was achieved. The results indicate that R12-AgNPs had low cytotoxicity and their potential suitability as a material for biomedical applications. The favorable biocompatibility of R12-AgNPs is an important finding, as it opens the door to various biomedical applications where these nanoparticles can be utilized without causing significant harm to living cells.