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People with obesity exhibit losses in muscle proteostasis that are partly improved by exercise training
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  • Kanchana Srisawat,
  • Connor Stead,
  • Katie Hesketh,
  • Mark Pogson,
  • Juliette Strauss,
  • Matt Cocks,
  • Ivo Siekmann,
  • Stuart Phillips,
  • Paulo Lisboa,
  • Sam Shepherd,
  • Jatin Burniston
Kanchana Srisawat
Liverpool John Moores University
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Connor Stead
Liverpool John Moores University
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Katie Hesketh
Liverpool John Moores University
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Mark Pogson
Liverpool John Moores University
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Juliette Strauss
Liverpool John Moores University
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Matt Cocks
Liverpool John Moores University
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Ivo Siekmann
Liverpool John Moores University
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Stuart Phillips
McMaster University
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Paulo Lisboa
Liverpool John Moores University
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Sam Shepherd
Liverpool John Moores University
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Jatin Burniston
Liverpool John Moores University

Corresponding Author:j.burniston@ljmu.ac.uk

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Abstract

This pilot experiment examines if a loss in muscle proteostasis occurs in people with obesity and whether endurance exercise positively influences either the abundance profile or turnover rate of proteins in this population. Men with (n = 3) or without (n = 4) obesity were recruited and underwent a 14-d measurement protocol of daily deuterium oxide (D2O) consumption and serial biopsies of vastus lateralis muscle. Men with obesity then completed 10-weeks of high-intensity interval training (HIIT), encompassing 3 sessions per week of cycle ergometer exercise with 1 min intervals at 100 % maximum aerobic power interspersed by 1 min recovery periods. The number of intervals per session progressed from 4 to 8, and during weeks 8-10 the 14-d measurement protocol was repeated. Proteomic analysis detected 352 differences (p < 0.05, false discovery rate < 5%) in protein abundance and 19 (p < 0.05) differences in protein turnover, including components of the ubiquitin-proteasome system. HIIT altered the abundance of 53 proteins and increased the turnover rate of 22 proteins (p < 0.05) and tended to benefit proteostasis by increasing muscle protein turnover rates. Obesity and insulin resistance are associated with compromised muscle proteostasis, which may be partially restored by endurance exercise.
18 Oct 2023Submitted to PROTEOMICS
27 Oct 2023Submission Checks Completed
27 Oct 2023Assigned to Editor
27 Oct 2023Review(s) Completed, Editorial Evaluation Pending
27 Oct 2023Reviewer(s) Assigned
03 Nov 2023Editorial Decision: Accept