Plants adapt to their local environment through complex interactions between genes, gene networks, and hormones. Although the impact of gene expression on trait regulation and evolution has been recognized for many decades, its role in the evolution of adaptation is still a subject of intense exploration. We used a Multi-parent Advanced Generation Inter-Cross (MAGIC) population, which we derived from crossing multiple parents from two distinct coastal ecotypes of an Australia wildflower, Senecio lautus. We focused on studying the contrasting gravitropic behaviors of these ecotypes, which have evolved independently multiple times and show strong responses to natural selection in field experiments, emphasizing the role of natural selection in their evolution. Here, we investigated how gene expression differences have contributed to the adaptive evolution of gravitropism. We studied gene expression in 600 pools at five time points (30, 60, 120, 240, and 480 minutes) after rotating half of the pools 90°. We found 428 genes with differential expression in response to the 90° rotation treatment. Of these, 81 genes (~19%) have predicted functions related to the plant hormones auxin and ethylene, which are crucial for the gravitropic response. By combining insights from Arabidopsis mutant studies and analyzing our gene networks, we propose a preliminary model to explain the differences in gravitropism between ecotypes. This model suggests that the differences arise from changes in the transport and availability of the hormones auxin and ethylene. Our findings indicate that the genetic basis of adaptation involves interconnected signaling pathways that work together to give rise to new ecotypes.