Quantification of malondialdehyde
Hepatic malondialdehyde (MDA) levels were measured using a lipid peroxidation assay kit (Abcam Inc., Cat# ab118970) according to the manufacturer’s protocol. Briefly, liver homogenates were mixed with thiobarbituric acid (TBA) to generate MDA-TBA adducts. The MDA-TBA adducts were then quantified colourimetrically.