Choline-deficient, L-amino acid defined, high-fat mouse model of NASH
C57BL/6J mice were randomized into groups of equal size and stratified into four groups according to body weight. The mice were fed a regular γ-ray-sterilized chow diet or CDAHFD and 3 or 10 mg・kg-1 CDDO-Me suspended in 0.5 w・v-1% methylcellulose (Fujifilm Wako Pure Chem. Corp.) or 0.5 w・v-1% methyl cellulose as the vehicle were orally administered every day to 8 mice in each group during the experiment. After 8 weeks of treatment with the regular diet or CDAHFD, all mice were weighed and euthanized under isoflurane anaesthesia. The livers were weighed and divided into sections, which were fixed in 10% phosphate-buffered formalin, frozen in liquid nitrogen, or placed in RNAlater solution (Thermo Fisher Scientific Inc., Waltham, MA) for further evaluation. Serum samples were collected and stored at -80 °C until further analyses.