Cell culture and in vitro treatment
Hepa1c1c7 cells (RRID:CVCL_0328) were obtained from DS Pharma Biomedical Co. Ltd. (Osaka, Japan), and maintained in MEMα containing 10% FBS and 1% penicillin/streptomycin/amphotericin B (Thermo Fisher Scientific Inc.). Hepa1c1c7 cells were seeded onto plates for 24 h and treated with the indicated concentrations of CDDO-Me for 6, 24, and 48 h. RAW264.7 cells (RRID:CVCL_0493) were obtained from the ECACC General Collection and maintained in DMEM containing 10% FBS and 1% penicillin/streptomycin/amphotericin B (Thermo Fisher Scientific Inc.). RAW264.7 cells were seeded onto plates for 24 h and treated with the indicated concentrations of CDDO-Me for 6 h. After incubation with CDDO-Me, cells were collected for quantitative PCR.