Figure 7. CDDO-Me inhibits recruitment of monocyte-derived macrophages in the liver of NASH mice
(a) Heatmap showing the expression of marker genes in each macrophage subtype previously identified using single-cell RNA-seq analysis. (b) Violin plot showing the expression of marker genes in the subtypes. Statistical differences were analysed using Dunnett’s multiple comparison test. (*P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001 versus vehicle-treated CDAHFD-fed mice). (c) Hepatic sections stained with anti-GPNMB, anti-CCR2, anti-SPN, or anti-CLEC4F antibodies. Scale bars represent 50 μm. (d-g) GPNMB (d), CCR2 (e), SPN (f), or CLEC4F (g)-positive cells quantified from the hepatic sections in Figure. 7c (mean + S.D., quadruplicate). Cont, Control; Veh, Vehicle. ns; not significant.