Lung function tests
Lung function tests were performed
using a FlexiVent instrument control ventilator (Montreal, Quebec,
Canada, SCIREQ, FlexiVent) with a forced oscillation system.
Specifically, a tracheotomy was performed on anesthetized mice, and a
spirometer was connected via a cannula. Pulmonary function parameters,
including inspiratory capacity (IC), forced vital capacity (FVC) and
forced expiratory volume in 100 ms (FEV100), as well as pulmonary
ventilation function tests such as airway resistance (RI), dynamic
compliance (Cdyn), chord compliance (Cchord), and mean mid-expiratory
flow (MMEF), were measured according to the standard operation of the
spirometer. All measures were assessed based on the mean of three
replicate values.