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Complete title: Value of a secretomic approach for distinguishing patients with COVID-19 viral pneumonia among patients with respiratory distress admitted to intensive care unit Shortened title: Secretomic to distinguish COVID-19 patients
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  • Angélique Blangy-Letheule,
  • Amandine Vergnaud,
  • Thomas Dupas,
  • Damien Habert,
  • Jérôme Montnach,
  • Walid Oulehri,
  • Dorian Hassoun,
  • Manon Denis,
  • Jules Lecomte,
  • Antoine Persello,
  • Antoine Roquilly,
  • José Courty,
  • Michel Seve,
  • Aurélia A. Leroux,
  • Bertrand Rozec,
  • Sandrine Bourgoin-Voillard,
  • Michel De Waard,
  • Benjamin Lauzier
Angélique Blangy-Letheule
Nantes Universite
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Amandine Vergnaud
Nantes Universite
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Thomas Dupas
Nantes Universite
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Damien Habert
Universite Paris-Est Creteil Val de Marne Faculte de medecine
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Jérôme Montnach
Nantes Universite
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Walid Oulehri
Les Hopitaux Universitaires de Strasbourg Hopital de Hautepierre
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Dorian Hassoun
Nantes Universite
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Manon Denis
Nantes Universite
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Jules Lecomte
Nantes Universite
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Antoine Persello
Nantes Universite
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Antoine Roquilly
Nantes Universite
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José Courty
Universite Paris-Est Creteil Val de Marne Faculte de medecine
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Michel Seve
Universite Grenoble Alpes Grenoble IAE
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Aurélia A. Leroux
Nantes Universite
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Bertrand Rozec
Nantes Universite
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Sandrine Bourgoin-Voillard
Universite Grenoble Alpes Grenoble IAE
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Michel De Waard
Nantes Universite
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Benjamin Lauzier
Nantes Universite

Corresponding Author:benjamin.lauzier@univ-nantes.fr

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Abstract

Introduction : In intensive care units, COVID-19 viral pneumonia patients (VPP) present symptoms similar to those of other patients with non-viral infection (NV-ICU). To better manage VPP, it is therefore interesting to better understand the molecular pathophysiology of viral pneumonia and to search for biomarkers that may clarify the diagnosis. The secretome being a set of proteins secreted by cells in response to stimuli represents an opportunity to discover new biomarkers. Aims : Identify secretomic signatures of VPP with those NV-ICU. Methods : Plasma samples and clinical data from NV-ICU (n=104), VPP (n=30) or healthy donors (HD, n=20) were collected at Nantes Hospital (France) upon admission. Samples were enriched for the low-abundant proteins and analyzed using non-target mass spectrometry. Specifically deregulated proteins (DEP) in VPP versus NV-ICU were selected. Combinations of 2 to 4 DEPs were established. Results : The differences in secretome profiles of the VPP and NV-ICU groups were highlighted. Forty-one DEPs were specifically identified in VPP compared to NV-ICU. Five combinations of 3 proteins with a receiver operating characteristic curve presenting an area under the curve of 95.0% were identified. Conclusion: This study identifies five combinations of candidate biomarkers in VPP compared to NV-ICU that may help distinguish the underlying causal molecular alterations.
18 Oct 2023Submitted to Journal of Medical Virology
18 Oct 2023Submission Checks Completed
18 Oct 2023Assigned to Editor
18 Oct 2023Review(s) Completed, Editorial Evaluation Pending
24 Oct 2023Reviewer(s) Assigned
29 Jan 2024Review(s) Completed, Editorial Evaluation Pending
06 Mar 2024Editorial Decision: Revise Minor
27 Mar 2024Reviewer(s) Assigned
10 Apr 2024Review(s) Completed, Editorial Evaluation Pending
12 Apr 2024Editorial Decision: Revise Minor
16 May 2024Reviewer(s) Assigned
05 Jun 2024Review(s) Completed, Editorial Evaluation Pending