Note: The dynamic process of sorting and precise positioning of nanoparticle biomass in pre-defined microstructures is very important, however, this is a major obstacle to the realization of surface-sensitive nanobiosensors and practical nanobiochips. A scalable, widespread and non-destructive trapping method based on dielectric forces is much needed for nanoparticle collection and nanobiosensing tools. Here, we present a vertical nanogap architecture with an electrode-insulator-electrode stack structure. Facilitate the generation of strong dielectric forces at low voltages, for precise capture and manipulation of nanoparticles and molecular assemblies, including lipid vesicles and amyloid-beta fibrillar proteins/oligomers. Our vertical nanoplastic platform allows low-voltage nanoparticles recorded in optical dimensional designs, providing new opportunities for the fabrication of advanced surface-sensitive sensors. Nano biosensors appear as a powerful alternative to conventional analytical techniques, as nanosensors perform highly sensitive, real-time, and high-frequency monitoring of pollutants without extensive sample preparation. Nano biosensors can be integrated into small devices for rapid screening and monitoring of a wide range of pollutants. Since the nano biosensor is an analytical device, used to detect a chemical substance, which is a combination of a biological component with a physicochemical detector. Sensitive biological element , for example tissue, micro-organisms , etc., component of material or biomimetic that interacts with nanoparticles.