DSS-induced colitis and design of drug treatment
C57BL/6 mice were fed with 3.0% (w/v ) DSS in drinking water for
7 days to induce acute colitis. In order to explore the impact of AB4 on
colitis, the mice were randomly divided into 5 groups (n =6 in
each group): Normal group, DSS group, 5-ASA (200 mg/kg) group, AB4
pretreatment group (5 mg/kg), and AB4 treatment group (5 mg/kg). To
further confirm the protective effect of AB4, the mice were randomly
divided into 6 groups (n =8 in each group): Normal group, DSS
group, AB4 (5, 10, and 15mg/kg) groups, and AB4 (15mg/kg) alone group.
To further confirmed that the protective effect of AB4 in DSS-induced
colitis is dependent on the intervention of NLRP3 inflammasome, WT and
NLRP3-/- mice were randomly divided into 6 groups (n=8
in each group): WT normal group, WT+DSS group, WT+DSS+AB4 (15mg/kg)
group, NLRP3-/- normal group,
NLRP3-/-+DSS group, NLRP3-/-+DSS+AB4
(15mg/kg) group.
To investigate whether the protective effect of AB4 against colitis
depends on the CD1d signaling pathway, the WT and
CD1d-/- mice were randomly divided into 6 groups (n=8
in each group): WT normal group; WT+DSS group; WT+DSS+AB4 (15mg/kg)
group; CD1d-/- normal group;
CD1d-/-+DSS group; CD1d-/-+DSS+AB4
(15mg/kg) group. Observe and record the changes in body weight, blood in
the stool, and diarrhea every day, and use a complete system to
calculate the DAI score(Cui et al., 2020; Lv et al., 2021).