FITC-dextran intestine-blood barrier tests
To analyze the permeability of the epithelial barrier in mice, on the 7th day, mice in each group were deprived of water and fasted for 4 h, and then each mouse was gavaged with FITC-dextran (0.6 mg/g; Sigma-Aldrich). The blood was collected after 4 h, and the content of FITC-dextran in serum was measured with a fluorescence spectrophotometer setup with an emission wavelength of 490 nm and an excitation wavelength of 520 nm.