Manganese concentration analyses
We explored Mn accumulation to better understand the effects of Mn
exposure on fish. Single organs (e.g., liver, brain) were too small to
provide sufficient dry weight for Mn detection. Instead, we focused on
the head (e.g., brain, gills) and the rest of the body (e.g., liver,
muscle tissue), i.e., we used two samples per fish in the analyses.
Samples were freeze dried for 48 hours (Christ Alpha 2–4), smashed and
digested in aqua regia (HNO3:HCl 1:3). Then, the digested tissue samples
were filtered and filled to a final volume of 10 ml with ultrapure
water, after which the samples were analyzed similarly than the water
samples with Perkin-Elmer Avio 500 ICP OES.