Author contributions
MB and JP conceived research and wrote the manuscript; MB carried out
the majority of the experiments, conception, design of the protocols,
analysis of the results and production of the figures. CC carried out G
protein sensor experiments, including protocol design, experiments and
analyses. NB performed theoretical and manual supervision of the
molecular biology experiments (electrophoresis, plasmid digestion,
transfection) and data interpretation. YC provided general assistance
with the acquisition and analysis of results in microscopy, he also
developed optimised programmes to facilitate and automate signal
analysis. JFI helped to design and perform experiments to detect mGlu5
receptor conformation. EM supervised and implemented neuronal cultures
with NB and the development of population BRET protocols. LT cloned the
pCAGGS-TRPC6-ires2-mbtdtomato plasmid. JC took an active part in the
implementation of the experimental protocol and together with LT
provided advices for patch clamp recordings on HEK and analysis of
results. JP carried out BRET and calcium microscopy experiments in
neurons, and was involved in the analysis of results and theoretical
supervision at all levels. JP conceived and supervised the full project.
All authors contribute to the preparation of the manuscript and approved
it.