Plasmids
Plasmids were amplified using E.Coli DH5α strain (Life Technologies).
DNA was then purified using the NucleoBond® Xtra Plus
Midi-Prep kit (Machery-Nagel®). The plasmids used
were: pcDNA3.1-rGFP-CAAX , pcDNA3.1-p63RhoGEF-RlucII , pcDNA3.1-Gαq
(gift from Jean-Philippe Pin), pRK5-SNAPTag-AT1 (CIS bio international),
pRK5-Myc-mGlu5a and pcDNA3.1-Myc-mGlu5a-Venus .
pCAGGS-TRPC6-ires2-mbtdtomato was obtained by cloning mouse TRPC6
sequence in pCAGGS plasmid upstream the sequences of IRES2 and
membrane-anchored tdtomato.