Fouad Kasim MohammadCollege of Veterinary Medicine, University of Mosul, Mosul, Iraqe-mail: fkmohammad@uomosul.edu.iq Chemicals: 7.1% aqueous solution of acetylcholine iodide, sodium barbital, potassium dihydrogen phosphate, sodium chloride, double distilled water.Barbital-Phosphate buffer: 1.237 g sodium barbital, 0.163 g potassium dihydrogen phosphate and 35.07 g sodium chloride/ L distilled water, pH 8.1.Equipment: Water bath, pH meterProcedure1. Collect venous blood samples in heparinized test tubes.2. Separate the plasma from erythrocytes by centrifugation.3. To a 10-ml beaker add 3 ml distilled water, 0.2 ml plasma, erythrocytes or uncentrifuged whole blood.4. Add 3 ml of barbital-phosphate buffer solution.5. Measure the pH of the mixture (pH1).6. Add 0.10 ml of 7.1% acetylcholine iodide to the mixture.7. Incubated at 37 ºC for 30 minutes.8. Measure the pH of the mixture (pH2).9. Calculated cholinesterase activity: Cholinesterase activity (DpH/30 min) = (pH1 – pH2) - D pH of blankNotes: The blank is without blood sample. The unit of activity is Δ pH/30 min. References and further reading1. Garmavy HMS, Mohammed AA, Rashid HM, Mohammad FK. Meta-analysis of normal human blood cholinesterase activities determined by a modified electrometric method. Journal of Medicine and Life 2023; 16(1):22-34.2. Mohammad FK, Garmavy HMS, Mohammed AA, Rashid HM. First meta-analysis study of cholinesterase inhibition in experimental animals by organophosphate or carbamate insecticides under the influence of diphenhydramine. Veterinary World 2023;16(1):118-125. 3. Mohammad FK. Clarifying an electrometric method for determining blood cholinesterase activity: a scientific letter. Asia Pacific Journal of Medical Toxicology 2022; 11(1):30-32.4. Mohammed AA, Mohammad FK. Recognition and assessment of antidotal effects of diphenhydramine against acute carbaryl insecticide poisoning in a chick model. Toxicology International 2022; 29(3): 339-352.5. Odisho SK, Mohammad FK. Blood cholinesterase activities and oxidative stress status among farmworkers using pesticides in Duhok, KRG, Iraq. Journal of Ideas in Health 2022;5(4):786-793.6. Mohammad FK, Al-Baggou BK, Naser AS and Fadel MA. In vitro inhibition of plasma and brain cholinesterases of growing chicks by chlorpyrifos and dichlorvos. Journal of Applied Animal Research 2014; 42: 423-8.
