ABSTRACT Candida albicans typically resides as a member of the indigenous vaginal microbiota and has been identified as the most common fungal pathogen responsible for vulvovaginal candidiasis, a condition that significantly impairs the well-being of women. Aloe barbadensis Miller, generally known as Aloe vera, has a long history of therapeutic use. The present study aimed to evaluate the antifungal properties of Aloe vera ethanol extract to develop an eco-friendly Aloe vera ethanolic gel treated cotton fabric against vulvovaginal candidiasis caused by Candida albicans. The antifungal activity of Aloe vera ethanol extract against Candida albicans was evaluated by the well diffusion method, and the highest concentration (5 mg/ml) showed a maximum mean zone diameter of 24.00±1.00 mm. Moreover, the minimum inhibitory concentration was recorded as 3.12 mg/ml using the broth macrodilution method. The cotton fabric (100% cotton) treated with 4 mg/ml of Aloe vera ethanol extract using the pad-dry-cure technique. The treated fabric exhibited a clear zone of inhibition against Candida albicans using the agar diffusion method. The wash durability test showed that treated cotton fabric retained antifungal activity up to 15 washing cycles with a fungal growth reduction rate of 15%. In conclusion, Aloe vera ethanol extract showed concentration dependent inhibitory activity on Candida albicans; furthermore, treated cotton fabric also exhibited effective antifungal activity against vulvovaginal candidiasis caused by Candida albicans in vitro.