4. Conclusion
This study proposes a novel formulation for the high-throughput cultivation medium of modified insulin, combining experimental methodologies using the BioLector microbioreactor and computational approaches employing DoE software. The efficacy of each step was validated by utilizing high-throughput PBD and CCD within the DoE software framework, complemented by the screening capabilities of the BioLector micro-bioreactor, enabling comprehensive assessments in a complex batch environment. The investigation primarily focused on Scenario-3, which exhibited superior attributes and demonstrated its potential as a more streamlined and cost-effective medium for achieving high cell density fermentation of the modified insulin in the E. coli Rosetta-2 strain.