4. Conclusion
This study proposes a novel formulation for the high-throughput
cultivation medium of modified insulin, combining experimental
methodologies using the BioLector microbioreactor and computational
approaches employing DoE software. The efficacy of each step was
validated by utilizing high-throughput PBD and CCD within the DoE
software framework, complemented by the screening capabilities of the
BioLector micro-bioreactor, enabling comprehensive assessments in a
complex batch environment. The investigation primarily focused on
Scenario-3, which exhibited superior attributes and demonstrated its
potential as a more streamlined and cost-effective medium for achieving
high cell density fermentation of the modified insulin in the E.
coli Rosetta-2 strain.