Figure 6. Administration of C-176 does not alter microglial
morphology 24h-post TBI.
30μm thick brain sections of CX3CR1eGFP mice subjected
to CCI TBI. Representative images are shown for the ipsilateral and
contralateral cortex of vehicle-treated (A) and C-176-treated(B) mice with an overview of the skeletonising process employed
using a minimum spanning tree algorithm to measure morphological
characteristics. Administration of C-176 did not significantly alter(C) total branch length (D), cell area or (E)soma area of perilesional microglia compared to vehicle treated mice
24h-post TBI. Each data point is an average of 200-500 cells. Scale bar
= 50μm. n=5 for each group.