In the present work, the epidermal mucus of Channa striatus ( C.striatus) stabilized the biosynthesis of zinc oxide nanoparticles (ZnO NPs) were synthesized. The nanoparticles were characterized using an Ultra-visible spectrophotometer (UV-Vis), Fourier transform infrared spectrophotometer (FT-IR), X-ray diffraction (XRD), and scanning electron microscope coupled with EDS. Various shapes like rod, hexagonal, A high-resolution transmission electron microscope (HR-TEM) reveal s the shape and selected area diffraction pattern. The samples were screened for toxicity using human cervical cancer cells (HeLa). The XRD pattern of mucus-stabilized ZnO NPs exhibits smaller size than that of the ZnO NPs without mucus. The anticancer efficiency of the ZnO NPs was examined by MTT assays in HeLa cells at different doses. ZnO NPs synthesized with 1 and 2 mL mucus exhibit 27.40 and 18.84 % of cell viability at 100 µg/ml. The microscopy results recommend that the synthesized ZnO NPs controlled the growth of the HeLa cells effectively at the concentration of 100 µg/mL. Therefore, mucus stabilized ZnO NPs can be used as an anticancer agents for the treatment of HeLa cells.