Breeding dose
The industry standard is to use 500 x 106progressively motile sperm for fresh AI and 1 x 109progressively motile sperm for cooled-shipped semen AI (Brinsko, 2006; Douglas-Hamilton et al., 1984; Pickett & Voss, 1975). In reality, some stallions can breed with lower a number of progressively motile sperm and still achieve acceptable pregnancy rates (Brinsko, 2006). Determining the minimum number of progressively motile sperm in a breeding dose for a particular stallion can be done through keeping detailed breeding records.