Simple Dilution – On Farm Use
For fresh semen the industry standard is to use 500 x
106 progressively motile sperm with semen extender
added in a 1:1 (semen: extender) ratio and maintain a volume less than
60-mL. This should then be inseminated into the mare after processing.
If there is a short delay from processing to insemination (<
12-hr) then the dose should be stored in a dark location at room
temperature until use (Love et al., 2002; Varner et al., 1989). Light
and incubator temperatures will adversely affect sperm longevity. If
there is a delay longer than 12-hr then the semen should be processed
for cooling and held cooled (~4oC)
until use.