Table 1. The minimum dilution ratio (semen: extender) for cooled-shipped semen based on the raw sperm concentration of semen and the progressive motility cutoff values for centrifugation by concentration of the ejaculate. If the raw semen sperm concentration is < 125 x 106 then centrifugation is needed, for other concentrations the minimum dilution ratio is listed in the adjacent column. In the table are specific concentrations for each range and the corresponding minimum progressive motility needed to avoid having an insemination dose with a volume > 60-ml. *If the progressive motility is less the cutoff value, centrifugation is recommended to avoid having a large insemination volume (> 60-ml). The progressive motility cutoff values are calculated for a breeding dose with a total number of progressively motile sperm of ≥ 1 x 109 and a volume of 60-ml.