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\begin{document}

\title{Processing stallion semen for on farm use and cooled transport}



\author[1]{Dale Kelley}%
\affil[1]{Texas A\&M University College Station}%


\vspace{-1em}



  
  \date{June 15, 2024}


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\selectlanguage{english}
\begin{abstract}
Simple dilution of semen is the oldest method to process semen for
artificial insemination with either fresh or cooled. This was developed
prior to the optimization centrifugation techniques for semen through a
combination of trial and error and research. Simple dilution and is a
faster method to process semen, is easy to use and works in many
circumstances. For on farm semen use a 1:1 (v/v) semen to extender ratio
is used. A variety of other techniques have been developed, such as
cushion centrifugation, density gradient centrifugation and sperm
filtration. Cushion centrifugation is used to concentrate the sperm and
allows higher centrifugation speeds than centrifugation alone. This
allows for improved recovery rates of sperm. Density gradient
centrifugation has been used to select for morphologically normal sperm
but suffers from low recovery rates. Processing sperm with this
technique can result in improved motility and improved DNA integrity of
sperm. Sperm filtration is another method to concentrate sperm without
the use of centrifugation; however, this technique has lower recovery
rates compared to cushion centrifugation. This review will discuss these
techniques in the application of processing equine semen for either
fresh or cooled-shipped use.%
\end{abstract}%



\sloppy


\textbf{Processing stallion semen for on farm use and cooled transport}

Dale E. Kelley

Department of Veterinary Clinical Sciences, College of Veterinary
Medicine, Oklahoma State University, Stillwater, Ok

\textbf{Summary}

Simple dilution of semen is the oldest method to process semen for
artificial insemination with either fresh or cooled. This was developed
prior to the optimization centrifugation techniques for semen through a
combination of trial and error and research. Simple dilution and is a
faster method to process semen, is easy to use and works in many
circumstances. For on farm semen use a 1:1 (v/v) semen to extender ratio
is used. A variety of other techniques have been developed, such as
cushion centrifugation, density gradient centrifugation and sperm
filtration. Cushion centrifugation is used to concentrate the sperm and
allows higher centrifugation speeds than centrifugation alone. This
allows for improved recovery rates of sperm. Density gradient
centrifugation has been used to select for morphologically normal sperm
but suffers from low recovery rates. Processing sperm with this
technique can result in improved motility and improved DNA integrity of
sperm. Sperm filtration is another method to concentrate sperm without
the use of centrifugation; however, this technique has lower recovery
rates compared to cushion centrifugation. This review will discuss these
techniques in the application of processing equine semen for either
fresh or cooled-shipped use.

\textbf{Introduction}

The ideal method for processing semen for use with either fresh or
cooled-shipped breeding would maximize the number or sperm per mL,
maximize total number of progressively motile sperm, minimize the volume
needed to breed a mare, not be deleterious to sperm function or
longevity and minimize seminal plasma. Based on these criteria, cushion
centrifugation is currently the best method. Cushion centrifugation
maximizes sperm recovery compared to sperm filters(Roach et al., 2016)
and centrifugation without cushion. Seminal plasma is removed, which can
be deleterious to sperm longevity(Braun et al., 1994; Jasko et al.,
1991), allows for the semen to be reconstituted more concentrated than
simple dilutions, which reduces the volume of the breeding dose while
increasing the number of sperm which inseminated into a mare.

Simple dilution of semen is perhaps the oldest method to extended semen
for artificial insemination with either fresh or cooled. This was
developed prior to the optimization centrifugation techniques for semen
through a combination of trial and error and research. Simple dilution
is a faster method to process semen, easy to use and works in many
circumstances. A variety of other techniques have been developed, such
as density gradient centrifugation, which can be used to remove abnormal
sperm and sperm filters which may be useful when a centrifuge is
unavailable. This review will discuss these techniques in the
application of processing equine semen for either fresh or
cooled-shipped use.

\textbf{Preparation}

This review will not go into depth on collection and evaluation of
semen; however, it is important to recognized that semen collection and
evaluation can greatly impact the quality of the semen after processing.
Two important factors in collecting semen which could impact the semen
quality are proper washing of the penis (Bowen et al., 1982; Dascanio,
2014) and lubrication of the artificial vaginal. Proper washing reduces
smegma in the ejaculate, which, even in small qualities, can be greatly
increased if centrifugation is required. Water is recommended for
washing, since detergents and disinfectants can increase the risk of
pathogenic bacteria colonizing the penis (Bowen et al., 1982).
Lubrication of the AV is important, but caution should be taken to
minimize the amount of lubricant to reduce contamination of the
ejaculate. A non-spermicidal lubrication should be used, but all
lubricants are spermicidal to some extent. Clarity brand lubrication has
been shown to have the least impact on sperm quality (Serafini et al.,
2019). Finally, if more than 2 collection attempts are required with an
artificial vagina (AV) to obtain an ejaculate, we recommend a replacing
the used AV with a clean, freshly prepared AV to reduce dirt and
bacterial contamination.

When evaluating semen, one should look at the color of the ejaculate and
a raw sample to determine the presence of sperm or other material such
as blood, white blood cells or urine crystals as these can not only
impact the accuracy of the evaluation but also the longevity of the
sperm. Concentration can be evaluated using a hemocytometer,
sphectrophotometer (Equine Densimeter, Spermacue\selectlanguage{ngerman}®, etc) or fluorescent
based system (nucleocounter®). Each system to measure concentration has
its advantages and disadvantages which should be recognized as this can
impact accuracy. The hemocytometer allows differentiation of cells
(sperm vs other) but is subject to human error either in dilutions,
counting sperm or mathematical mistakes. A spectrophotometer-based
system works will in situations with little contamination of the
ejaculate that is reading on the middle of the standard curve. This
system will overestimate sperm numbers if debris, red blood cells, urine
or other material is present such as extenders. Additionally, this
system is not accurate in cases of low sperm concentration or high sperm
concentration. Fluorescent-based systems stain the DNA of the sperm.
Thus, non-cellular or red blood cell contamination will not interfere
with the accuracy. In cases of pyospermia, this system will overestimate
sperm numbers. In situations where the spectrophotometer and
fluorescent-based systems are inaccurate, we recommend evaluation with a
hemocytometer to try to gain a more accurate sperm concentration. For
more in-depth reviews on semen processing and evaluation please see
(Jasko, 1992; Love, 2016).

\textbf{Semen Extender}

Several commercial extenders are available for processing semen for both
on farm and cooled shipping. It is beyond the scope of this review to
discuss the composition and function of extenders and several reviews
are available (Aurich, 2008; Brinsko \& Varner, 1992; Gibb \& Aitken,
2016) .

\textbf{Breeding dose}

The industry standard is to use 500 x 10\textsuperscript{6}progressively
motile sperm for fresh AI and 1 x 10\textsuperscript{9}progressively
motile sperm for cooled-shipped semen AI (Brinsko, 2006;
Douglas-Hamilton et al., 1984; Pickett \& Voss, 1975). In reality, some
stallions can breed with lower a number of progressively motile sperm
and still achieve acceptable pregnancy rates (Brinsko, 2006).
Determining the minimum number of progressively motile sperm in a
breeding dose for a particular stallion can be done through keeping
detailed breeding records.

\textbf{Insemination volume}

An interaction between the total number of sperm inseminated, volume
inseminated and sperm concentration has been suggested (Pickett \&
Shiner, 1994) and sperm concentration may be more important than volume
(Katila, 2005). When insemination of mares with a constant total number
of sperm, larger volumes reduce pregnancy rates (Jasko, Martin, et al.,
1992; Pickett \& Shiner, 1994; Rowley et al., 1990), however when the
concentration of sperm was consistent (50 x
10\textsuperscript{6}sperm/ml) there was no difference in pregnancy rate
between 30-ml and 120-ml (Bedford \& Hinrichs, 1994) of semen. Large
insemination volumes (\textgreater{} 50-mL) are not thought to be
beneficial as most of the semen will be lost through the mares dilated
cervix post insemination (Brinsko et al., 2011; Jones, 1995; Rowley et
al., 1990). When considering the inflammation in the mare's uterus
post-breeding, the data is confounding. Two studies have found an
increase in uterine inflammation with increased sperm
concentration(Fiala et al., 2007; Kotilainen et al., 1994). Another
study found no effect of sperm concentration on uterine inflammation
(Sinnemaa et al., 2005) while a second study found mares infused with
40-mL of extended semen with a concentration of 500 x
10\textsuperscript{6} sperm/mL and significantly less uterine
inflammation than those infused with 40-mL at a concentration of 50 x
10\textsuperscript{6}(Nikolakopoulos \& Watson, 2000). Thus, it is this
author's recommendation to minimize the insemination volume while having
enough sperm to produce acceptable pregnancy rates.

\textbf{Centrifugation for concentrating sperm}

Centrifugation can be performed either with or without cushion solution.
Cushion is an iodixanol solution which allows the use of higher
centrifugation g-forces which reduces sperm damage and improves sperm
recovery rates. After semen is analyzed, it is diluted 1:1 using a semen
extender. After the semen is diluted, it is transferred to 50-mL conical
tubes. If cushion is not being used, the tubes are balanced and
centrifuged at 400 x g for 20 minutes. If cushion is being used, after
the diluted semen is added to the 50-mL conical tubes, the cushion
solution is layered underneath the diluted semen. The amount of cushion
solution used can vary on user preference and stallion but is typically
between 0.5-mL and 3-mL. The layering of cushion is done by first
drawing the cushion solution into syringes and the appropriate volume.
Next, a Tom-cat catheter, pipette, or blunted spinal needle can be used
to deposit the cushion under the diluted semen. Once this is done the
tubes are weighed and balanced and centrifuged at 1000 x g for 20
minutes. It important for the centrifuge to be well balanced as any
vibration can disrupt the sperm pellet and reduce the recovery rate of
sperm. After centrifugation, the supernatant is removed. This can be
done with a pipette or a vacuum aspirator. It is important to remove the
supernatant as soon as possible after centrifugation to maximize the
sperm recovery rate. Next, the samples are combined and resuspended into
one tube and a concentration and volume measured. When using
centrifugation to process semen the reconstituted volume can be much
lower and sperm concentration much higher than using simple dilution
without reducing sperm longevity. Research has found difference in sperm
longevity after cushion centrifugation in sperm undergoing
cooled-storage at 25 x 10\textsuperscript{6} sperm/mL and 250 x
10\textsuperscript{6} sperm/mL (Bliss et al., 2012). There is an
excellent review on processing semen from subfertile stallions (Varner,
2016) which covers cushioned centrifugation techniques in more detail.

\textbf{Simple Dilution -- On Farm Use}

For fresh semen the industry standard is to use 500 x
10\textsuperscript{6} progressively motile sperm with semen extender
added in a 1:1 (semen: extender) ratio and maintain a volume less than
60-mL. This should then be inseminated into the mare after processing.
If there is a short delay from processing to insemination (\textless{}
12-hr) then the dose should be stored in a dark location at room
temperature until use (Love et al., 2002; Varner et al., 1989). Light
and incubator temperatures will adversely affect sperm longevity. If
there is a delay longer than 12-hr then the semen should be processed
for cooling and held cooled (\textasciitilde{}4\textsuperscript{o}C)
until use.

\textbf{Simple Dilution -- Cooled-Shipped Semen}

The general guidelines for extending semen for cooled-shipping are to
have between 5\% to 20\% seminal plasma (Jasko, Hathaway, et al., 1992),
a concentration of 25 x 10\textsuperscript{6} to 50 x
10\textsuperscript{6} sperm/mL (Varner et al., 1987), a volume less than
50-mL (Loomis, 1992) and 1 x 10\textsuperscript{9} progressively motile
sperm in the final breeding dose.

Semen extended with 5 to 20\% seminal plasma had better motility at 24,
48 and 72 hours of cooled storage (Jasko, Hathaway, et al., 1992). This
equates to a dilution ration (semen: extender) from 1:4 to 1:19. Thus,
the minimum recommended dilution ratio is 1:4 for cooled-shipped semen.
The final extended concentration of sperm should be between 25 to 50 x
10\textsuperscript{6} sperm/mL. Dilution of semen to 25 x
10\textsuperscript{6} sperm/ml resulted in highest total and progressive
motility after 24 h compared to 50 x 10\textsuperscript{6} , 100 x
10\textsuperscript{6} and no dilution, with the 50 x
10\textsuperscript{6} having the second best motility values (Varner et
al., 1987). Lastly, The standard recommendation iis to ship \selectlanguage{english}[?] 1 x
10\textsuperscript{9} progressively motile sperm in the insemination
dose (Brinsko, 2006) and minimize the insemination volume ( keep
it\emph{\textless{}} 50-mL).

There are situations when these guidelines cannot be met such as when a
stallion has poorly concentrated semen or poor progressive motility. In
these situations, the semen should be concentrated using a technique
such as cushion centrifugation, to reduce the total volume while
maintaining an acceptable number of progressively motile sperm in the
breeding dose. A simplified table (Table 1) was developed to assist in
determining the minimum dilution ratio (semen: extender) for
cooled-shipped semen based on the raw sperm concentration of semen and
the progressive motility cutoff values for centrifugation by sperm
concentration in the ejaculate.

\textbf{Other Semen Processing Techniques}

Continuous density gradient centrifugation (also known as single layer
colloid) can be a useful technique in some stallions to improve the
sperm morphological profile, motility and DNA integrity post processing
(Edmond, 2010). One commercially available product is
EquiPure\textsuperscript{TM}. It is important to note that this
technique does not improve the post processing sperm quality in all
stallions, and that a significant number of sperm are lost. Common
recovery rates with density gradient centrifugation are from 30\% to
40\% but can be much lower (Edmond et al., 2012). Thus, it is important
to test the effectiveness of this technique on any given stallion prior
to shipping semen to mares, by not only looking at the recovery rate but
also the morphological profile of sperm after the use of density
gradient centrifugation.

Briefly, for this technique semen is extended 1:1 (v/v) with pre-warmed
extender. Using either 15-ml or 50-ml conical tubes, the density
gradient media (EquiPure\textsuperscript{TM}) is added to the tube
first. The volume of EquiPure\textsuperscript{TM} used is typically
equal to the amount of extended semen to be added. Next, the extended
semen is then added to form a layer on top being careful not to mix the
extended semen with the colloid. The tubes are then centrifuged at 400 X
g for 20 minutes and afterwards the supernatant removed, and the pellet
resuspended. For more details on the procedure the reader is referred to
(Bradecamp, 2014a).

Another method to concentrate sperm without the use of centrifugation is
through filtration. The recovery rate of sperm using this method was
originally reported at 92\% and 95\%, depending on the size of the pores
in the filter (Alvarenga et al., 2010). The sperm recovery rate of the
sperm filter is lower compared to cushion centrifugation and differences
in motility between the two techniques were negligible, with the authors
suggesting this may be a useful technique when centrifugation is
unavailable (Roach et al., 2016).

When using the SpermFilter, a petri dish is needed and the semen is
extended 2:1 (semen:extender, v/v). The extended semen is added on top
of the SpermFilter while it is gently swirled allowing fluid to flow
through the filter, collecting sperm on top. Once the sperm has been
filtered the membrane is washed with a predetermined volume into a
separate petri dish to recover the sperm. For more details please see
(Alvarenga \& Segabinazzi, 2021).

\textbf{Packaging and shipping cooled semen}

To ensure sperm survival proper packaging is critical. There are several
excellent reviews on semen packaging (Aurich, 2008; Bradecamp, 2014b)
and thus only certain important points will be highlighted. For
packaging, several types of containers can be used such as Whirl-Pak(r)
bags, latex/rubber free syringes, conical tubes or screw top tubes. The
important point is the storage device does not leak during
transportation. Traditionally, it was recommended to remove the air in
stored extended semen; however this may not be critical as similar
motility parameters and viability have been seen under aerobic and
anaerobic conditions (Price et al., 2008). Next, there are many
commercial cooled shipping containers. It is recommended to follow the
manufacturer's directions for packaging. Finally, make sure to clearly
label the packaged semen and include easily identified documentation of
the stallion, date and time of collection, sperm concentration, volume
of extended semen and motility of the sperm being shipped within the
container.

Acknowledments

I would like to thank G. Reed Holyoak for taking time to proof read this
manuscript.

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15degC. \emph{Reproduction in Domestic Animals} , \emph{43} (3), 261-266.
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Roach, J., Schnobrich, M., Ellerbrock, R., Feijo, L., Bradecamp, E.,
Alvarenga, M. A., Kline, K., \& Canisso, I. (2016). Comparison of
cushioned centrifugation and SpermFilter filtration on longevity and
morphology of cooled-stored equine semen. \emph{Veterinary Record}
,\emph{178} (10), 241-241.
https://doi.org/https://doi.org/10.1136/vr.103607

Rowley, H. S., Squires, E. L., \& Pickett, B. W. (1990). Effect of
insemination volume on embryo recovery in mares. \emph{Journal of Equine
Veterinary Science} , \emph{10} (4), 298-300.
https://doi.org/https://doi.org/10.1016/S0737-0806(06)80014-6

Serafini, R., Ghosh, S., Love, C. C., Medrano, J. M. R., Teague, S. R.,
LaCaze, K. A., \& Varner, D. D. (2019). Effect of artificial vagina
lubricants on stallion sperm quality. \emph{Theriogenology} , \emph{139}
, 121-125.
https://doi.org/https://doi.org/10.1016/j.theriogenology.2019.08.004

Sinnemaa, L., Jarvimaa, T., Lehmonen, N., Makela, O., Reilas, T.,
Sankari, S., \& Katila, T. (2005). Effect of Insemination Volume on
Uterine Contractions and Inflammatory Response and on Elimination of
Semen in the Mare Uterus -- Scintigraphic and Ultrasonographic
Studies.\emph{Journal of Veterinary Medicine Series A} , \emph{52} (9),
466-471.
https://doi.org/https://doi.org/10.1111/j.1439-0442.2005.00757.x

Varner, D. D. (2016). Strategies for Processing Semen from Subfertile
Stallions for Cooled Transport. \emph{Veterinary Clinics: Equine
Practice} , \emph{32} (3), 547-560.
https://doi.org/10.1016/j.cveq.2016.07.007

Varner, D. D., Blanchard, T. L., Love, C. L., Garcia, M. C., \& Kenney,
R. M. (1987). Effects of semen fractionation and dilution ratio on
equine spermatozoal motility parameters. \emph{Theriogenology}
,\emph{28} (5), 709-723.
https://doi.org/https://doi.org/10.1016/0093-691X(87)90288-3

Varner, D. D., Blanchard, T. L., Meyers, P. J., \& Meyers, S. A. (1989).
Fertilizing capacity of equine spermatozoa stored for 24 hours at 5 or
20degC. \emph{Theriogenology} , \emph{32} (4), 515-525.
https://doi.org/https://doi.org/10.1016/0093-691X(89)90273-2

\textbf{Table 1.} The minimum dilution ratio (semen: extender) for
cooled-shipped semen based on the raw sperm concentration of semen and
the progressive motility cutoff values for centrifugation by
concentration of the ejaculate. If the raw semen sperm concentration is
\textless{} 125 x 10\textsuperscript{6} then centrifugation is needed,
for other concentrations the minimum dilution ratio is listed in the
adjacent column. In the table are specific concentrations for each range
and the corresponding minimum progressive motility needed to avoid
having an insemination dose with a volume \textgreater{} 60-ml.
*\emph{If the progressive motility is less the cutoff value,
centrifugation is recommended to avoid having a large insemination
volume (\textgreater{} 60-ml).} The progressive motility cutoff values
are calculated for a breeding dose with a total number of progressively
motile sperm of \selectlanguage{english}[?] 1 x 10\textsuperscript{9} and a volume of 60-ml.\selectlanguage{english}
\begin{longtable}[]{@{}llllllllllllllllllllllllllll@{}}
\toprule
\begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
\textbf{Raw} \textbf{Concentration} \textbf{Range} \textbf{(x
10\textsuperscript{6} sperm/ml)}\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
\textbf{Dilution} \textbf{ratio} \textbf{(Semen: extender)}\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage} & \begin{minipage}[b]{0.03\columnwidth}\raggedright\strut
Specific Sperm Concentrations of Raw Semen\strut
\end{minipage}\tabularnewline
\midrule
\endhead
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{\textless{} 125}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{Centrifuge}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{125 - 250}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{1:4}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{125}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{130}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{135}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{140}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{145}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{150}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{155}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{160}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{165}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{170}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{175}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{180}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{185}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{190}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{195}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{200}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{205}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{210}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{215}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{220}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{225}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{230}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{235}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{240}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{245}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{250}\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{250 - 300}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{1:5}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{252}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{258}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{264}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{270}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{276}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{282}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{288}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{294}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{300}\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{300 - 350}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{1:6}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{301}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{308}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{315}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{322}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{329}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{336}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{343}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{350}\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{350 - 400}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{1:7}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{352}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{360}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{368}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{376}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{384}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{392}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{400}\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{400 - 450}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{1:8}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{405}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{414}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{423}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{432}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{441}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{450}\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{450 - 500}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{1:9}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{460}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{470}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{480}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{490}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{500}\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{500-550}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{1:10}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{506}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{517}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{528}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{539}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{550}\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{550-600}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{1:11}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{552}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{564}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{576}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{588}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{600}\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{600 - 650}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{1:12}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{611}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{624}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{637}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{650}\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{650 - 700}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{1:13}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{658}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{672}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{686}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{700}\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{700 - 750}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{1:14}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{705}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{720}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{735}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\emph{750}\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{Progressive Motility}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
Cutoff (\%) *\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{Progressive Motility}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
Cutoff (\%) *\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{67}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{65}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{62}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{60}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{58}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{56}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{54}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{53}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{51}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{49}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{48}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{47}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{45}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{44}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{43}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{42}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{41}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{40}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{39}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{38}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{37}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{37}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{36}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{35}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{34}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{34}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{Extended Concentration}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
(x10\textsuperscript{6} sperm/ml)\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{Extended Concentration}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
(x10\textsuperscript{6} sperm/ml)\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{25}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{26}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{27}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{28}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{29}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{30}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{31}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{32}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{33}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{34}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{35}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{36}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{37}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{38}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{39}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{40}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{41}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{42}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{43}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{44}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{45}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{46}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{47}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{48}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{49}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\textbf{50}\strut
\end{minipage} & \begin{minipage}[t]{0.02\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\bottomrule
\end{longtable}

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\end{document}

