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\begin{document}

\title{Evaluation of has-circ-00001724 gene expression in breast cancer
patients and its relationship to disease prognosis}



\author[1]{Alireza Yousefi Ladmakhi}%
\author[1]{Abolhassan Rezaei}%
\author[2]{masoud mortezazadeh}%
\author[3]{Seyyed taher  Seyyed mahmoudi}%
\author[4]{ehsan kamali yazdi}%
\author[5]{abbas mofidi}%
\author[6]{ensieh sadat mansouri}%
\author[2]{Mehdi Kashani}%
\author[7]{Hossein Mofidi}%
\author[8]{Niyousha Shirsalimi}%
\affil[1]{Islamic Azad University Tonekabon Branch}%
\affil[2]{Tehran University of Medical Sciences}%
\affil[3]{Tabriz University of Medical Sciences}%
\affil[4]{hematology and oncology department, Imam khomeini hospital complex, tehran university of medical sciences}%
\affil[5]{Iran University of Medical Sciences}%
\affil[6]{Internal Medicine Department, Sina Hospital, Tehran University of Medical Science, Tehran, Iran}%
\affil[7]{Faculty of Industrial Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran}%
\affil[8]{Hamadan University of Medical Sciences}%


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  \date{June 13, 2024}


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\begin{abstract}
Introduction: Breast cancer is a leading cause of cancer-related deaths
in females{[}1{]}, and circular RNAs (circRNAs) have emerged as a novel
class of noncoding RNAs that play regulatory roles in angiogenesis and
cancer progression. The study of the expression patterns and functional
roles of circRNAs in breast cancer has become an area of growing
interest. The aim of this study was to investigate the relationship
between hsa-circ-00001724 gene expression and breast cancer. method:
Circular RNAs were extracted from tissue samples and cDNAs were
synthesized, followed by RT-PCR of the glyceraldehyde-3-phosphate
dehydrogenase gene as an internal control to ensure the quality of the
synthesized cDNA samples. Negative controls were used in RT-PCR and
qRT-PCR to exclude contamination with genomic DNA and PCR materials, and
a positive control test was also performed. The most appropriate primer
sequences were selected using OligoAnalyzer software and the NCBI
website, and real-time PCR using SYBR Green was used to examine
expression changes. The CT number was determined from the data obtained,
and the resulting graphs were examined in terms of dimer formation. The
Ct values of normal and tumor samples of the CIRC gene were also
subjected to a parametric Kolmogorov-Smirnov test. Results: Real-time
PCR reaction results revealed that the hsa-circ-00001724 gene had lower
expression in cancer tumor samples than in non-tumor samples.
Conclusion: Based on these results, it is hypothesized that
hsa-circ-00001724 may serve as a potential biomarker for breast cancer
diagnosis and prognosis, and may also be a potential target for
therapeutic purposes.%
\end{abstract}%



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\textbf{Hosted file}

\verb`Evaluation of has-circ-00001724 gene expression in breast cancer patients and its relationship to disease prognosis (final).docx`  available at \url{https://authorea.com/users/643426/articles/656797-evaluation-of-has-circ-00001724-gene-expression-in-breast-cancer-patients-and-its-relationship-to-disease-prognosis}

\textbf{Hosted file}

\verb`tables.docx`  available at \url{https://authorea.com/users/643426/articles/656797-evaluation-of-has-circ-00001724-gene-expression-in-breast-cancer-patients-and-its-relationship-to-disease-prognosis}

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