PHYSICAL METHODS
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Adsorption
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-Simple
-No reagents are required
-Low cost
-No modification of the enzyme
-Support reusability
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-Enzyme desorption
-Low stability
-Low efficiency
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Encapsulation
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-Simple
-Low cost
-Enzymes are stable for long time
-Native conformation of the enzyme is kept
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-Mass transfer limitations of the active site
-Enzyme leakage
-High enzyme concentration required
-Pore size limitations
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Entrapment
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-Simple and fast
-Low cost
-High stability
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-Enzyme leakage
-Difficult to implement at industrial level
-Pore diffusion restraint
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CHEMICAL METHODS
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Covalent binding
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-Prevents leaking
-High heat stability
-Strong binding
-Facilitates the contact between enzyme and substrate
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-Expensive
-Complex method
-Loss of activity
-Chemical enzyme modification
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Crosslinking
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-Strong binding
-No carrier needed
-Increases stability
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-Might cause alteration in the active site of the enzyme
-Crosslinking reagent is needed
-Loss of activity
-Diffusion limitations
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