Advantages Drawbacks
PHYSICAL METHODS
Adsorption
-Simple -No reagents are required -Low cost -No modification of the enzyme -Support reusability -Enzyme desorption -Low stability -Low efficiency
Encapsulation
-Simple -Low cost -Enzymes are stable for long time -Native conformation of the enzyme is kept -Mass transfer limitations of the active site -Enzyme leakage -High enzyme concentration required -Pore size limitations
Entrapment
-Simple and fast -Low cost -High stability -Enzyme leakage -Difficult to implement at industrial level -Pore diffusion restraint
CHEMICAL METHODS
Covalent binding
-Prevents leaking -High heat stability -Strong binding -Facilitates the contact between enzyme and substrate -Expensive -Complex method -Loss of activity -Chemical enzyme modification
Crosslinking
-Strong binding -No carrier needed -Increases stability -Might cause alteration in the active site of the enzyme -Crosslinking reagent is needed -Loss of activity -Diffusion limitations