Analysis of OXP1 transcript level in CsGGCT2;1overexpression lines
WT and CsGGCT2;1 OE lines were grown for 21 days on ½ x MS and ½
x MS media supplemented with AsIII (20 µM). After RNA isolation and cDNA
synthesis, qRT-PCR was performed using CsOXP1 forward (5’
GTCTTCTGCGTTGACACCCA 3’) and reverse primers (5’ GGTGCCAAGCCTCCATCAGA
3’). CsEF1 (housekeeping) primers and qRT-PCR conditions were the
same as mentioned earlier.