Differential expression of CsGGCT2;1 gene
To gain insight into the potential function of CsGGCT2;1 for arsenic tolerance or sensitivity in Camelina, its differential regulation was analyzed in root and shoot tissues exposed to AsIII. Camelina wild-type plants grown in Hoagland’s solution and exposed to AsIII for 0, 6, 12, 24, and 48 hours were used for the differential regulation of CsGGCT2;1 transcripts using qRT-PCR. The results show that the CsGGCT2;1 gene was induced after 6 h of exposure to AsIII in both root and shoot (Figure 2). In shoots, the expression level at 6 h showed a 3-fold increase and then decreased to control levels after 48 h exposure (Figure 2a). In roots, there was a 17-fold increase in the expression of CsGGCT2;1 after 6 h of AsIII exposure, that then waned to a 6-fold difference at 12 h, and then again increased to 15-fold difference at 48 h (Figure 2b).