ROS analysis in Camelina seedlings
ROS was analyzed in Camelina WT and CsGGCT2;1 OE lines grown on ½ x MS and AsIII treatments using H2DCF-DA staining. The seedlings were grown for two weeks on the media, and the root tips were stained with H2DCF-DA, washed with DI water, and then observed under confocal fluorescent microscopy. As depicted by fluorescence, WT roots had significantly higher ROS under AsIII treatments as compared to the CsGGCT2;1 OE lines (Figure 4c). Based on quantification of fluorescence, there was a significant (~ 40%) reduction in fluorescence in CsGGCT2;1OE lines than in the WT plants (Figure 4d).