Background While the Pediatric Quality of Life Inventory™ (PedsQL™) 3.0 eosinophilic esophagitis (EoE) Module is widely used for EoE assessment, no comprehensive transcultural adaptation has been completed, except for one linguistic validation study in Spanish. To assess the transcultural adaptation of the Turkish version of PedsQL™ (Tr-PedsQL™) 3.0 EoE Module for teens and parent reports through validity and reliability tests, marking a first in the field. Methods Teens with EoE and their parents were included in the study. Linguistic validation, content validity (CnV) and field test for construct validity (CsV) and reliability were completed in the adaptation of the Tr-PedsQL™ 3.0 EoE Module. Convergent and divergent validity (CgV and DgV) were examined for CsV by correlation analysis between Turkish version of Pediatric EoE Symptom Scores® (Tr-PEESS) v2.0 and Tr-PedsQL ^TM 3.0 EoE Module scores. Reliability was determined through internal consistency (Cronbach-α) and test-retest reliability (intraclass correlation coefficients: ICC). Results Thirty-three teens and their parents completed the study. CnV indexes were > 0.8 for all items. Good correlations between Tr-PEESS v2.0 and Tr-PedsQL ^TM 3.0 EoE Module Total, Symptoms I, II and Total scores substantiated CgV, while low or absent correlations in certain dimensions evidenced DgV. Tr-PedsQL ^TM 3.0 EoE Module showed good internal consistency (Cronbach-α: 0.61-0.90) and good to excellent test-retest reliability (ICC: 0.713-0.935). Conclusions This study is the first to adapt the PedsQL™ 3.0 EoE Module for another language with its validity and reliability in assessing the health-related quality of life among Turkish-speaking teens with EoE and their parents.