Supplementary Figure 5. SAHA steadily increases transgenic
expression over culture period.
Flask liquid cultures of clones 16, 17, 18, 20, 21 and WT were treated
with 5 mM of SAHA and grown for 12 days. The OD750 and
chlorophyll fluorescence (RFU) were assessed every day. A. The
OD750 was measured on the overall culture using a
microplate reader. B. The chlorophyll fluorescence (RFU) was measured on
the overall culture using a microplate reader. Statistical analysis:
Dunnett’s multiple comparisons test, analyzing OD750,
there was a statistically significance difference for clone 18: DMSO vs.
SAHA ** p= 0.0088. Upon analyzing chlorophyll fluorescence, there
were statistically significant differences comparing DMSO with SAHA
treatment for WT (p= 0,0054), for clone 16 (p= 0,0046),
clone 17 (p= 0,0080), clone 18 (p= 0,0057), clone 20
(p= 0,0151), clone 21 (p= 0,0018).