Figure 5. SAHA induces a palmelloid phenotype in C. reinhardtii.
A. SAHA’s effect on cell viability (membrane integrity) was verified using propidium iodide (PI) staining and analyzed on a flow cytometer (FL3 channel). B-D. Representative microscopy pictures of liquid culture in 96 wells of transformants treated with DMSO, 5 and 10 µM of SAHA (respectively) for 6 days. E. Bar graph with means % of palmelloids in transformants following 5 µM SAHA treatment at day 3 and 6, as measured on a flow cytometer. FSC: forward scatter, SSC Side scatter. Error bars represent standard deviation of biological triplicates. Two-way ANOVA repeated value statistical test was used, ***p < 0.001, **p <0.01 (effect of SAHA on palmelloid formation).