Supplementary Figure 5. SAHA steadily increases transgenic expression over culture period.
Flask liquid cultures of clones 16, 17, 18, 20, 21 and WT were treated with 5 mM of SAHA and grown for 12 days. The OD750 and chlorophyll fluorescence (RFU) were assessed every day. A. The OD750 was measured on the overall culture using a microplate reader. B. The chlorophyll fluorescence (RFU) was measured on the overall culture using a microplate reader. Statistical analysis: Dunnett’s multiple comparisons test, analyzing OD750, there was a statistically significance difference for clone 18: DMSO vs. SAHA ** p= 0.0088. Upon analyzing chlorophyll fluorescence, there were statistically significant differences comparing DMSO with SAHA treatment for WT (p= 0,0054), for clone 16 (p= 0,0046), clone 17 (p= 0,0080), clone 18 (p= 0,0057), clone 20 (p= 0,0151), clone 21 (p= 0,0018).