The genus Orthoflavivirus integrates several arboviruses of great im- portance in public health, including West Nile virus (WNV). The search for new bioactive compounds of natural origin has become a very important line of research. The species Inga edulis Martius is used in folk medicine as an antidiarrheal and anti-inflammatory agent. In silico analyses were performed to predict the pharmacokinetics and toxicodynamics of the main compounds present in the extract, followed by in vitro analyses. A time-of-addition assay was carried out to evaluate the antiviral effect of the extract at different stages of infection. The results demonstrate the main compounds of the extract did not retain the blood-brain barrier permeability and their bioavailability complied with what is expected for a good drug in some parameters. The CC50 obtained was 144.6 µg/mL, with the highest concentrations used (25 and 50 µg/mL) not significantly cytotoxic to the cells. The concentration of 25 µg/mL showed anti-WNV action when added before or during the infection procedure. It is therefore concluded that the compounds from the crude extract of I. edulis leaves have an inhibitory activity in WNV infection in neuroblastoma cells and can be further explored for the development of antiviral drugs.