Detection of plasmid specificity
We synthesized mutant plasmids of CYP2C19*4, CYP2C19*5, CYP2C19*6, CYP2C19 *7, CYP2C19 *8 and CYP2C9, as shown in Figure 6. The red marker indicates the SNP site of the gene, and the plasmid was diluted with 1X TE solution to a concentration of 103 copies/µl. The results were analyzed by the RPCR-MC detection system. Only CYP2C19 * 2/3/17 heterozygous mutant samples were detected, and each site had a single correct Tm value; Conversely, the other types of samples were not detected, showing that the detection system we established has good specificity.