Figure 6 . Cytotoxicity of unfunctionalized VLP-GOx and functionalized VLP-GOx@HSA nanoreactors on 293T kidney cell line.
The cytotoxicity was also assayed in 293T healthy embryonic kidney human cells (Fig. 6). The results were expressed as both GOx protein content and nanoreactor GOx activity. The LD50 values were 0.45 µg GOx/mL for VLP-GOx and around 0.79 µg GOx/mL for VLP-GOx@HSA, and no metabolic activity was detected at 1 µg GOx/mL for VLP-GOx preparation (Fig. 6a). The VLP-GOx nanoreactors on 293T kidney cells showed an LD50 at around 15 mU of GOx activity per mL of cell culture, and no metabolic activity at around 30 mU/mL (Fig. 6b). On the other hand, the VLP-GOx@HSA nanoreactors showed an LD50around 8 mU/mL (Fig. 6b).