2.6 GOx Nanoreactors Characterization
The nanoreactor catalytic activity was characterized by a peroxidase-coupled reaction followed by a colorimetric assay. The last product in this coupled reaction is tetraguaiacol, which absorbs at 470 nm. The increased absorbance is directly related to the glucose transformation rate. A Michaelis-Menten regression was performed by preparing reactions at different glucose concentrations (0, 25, 50, 100, and 150 mM) to describe the catalytic parameters of the VLP-GOx nanoreactors.
The encapsulation of the GOx enzyme in VLP nanoparticles and the HSA coating was confirmed by SDP-PAGE. Finally, the size and morphology of the nanoreactor were studied with transmission electron microscopy (TEM, JEOL-2010, JEOL) operated at 100 kV.