Figure 7. Fluorescence microscopy images of the triple-negative
breast cancer cell line MDA-MB-231 expressing GFP. The images were taken
at different times after treatment with VLP-GOx@HSA. The bubble-like
formations are shown with yellow arrows. See the videos in the
Supplementary information.
The effect of the treatment with VLP-GOx@HSA on a triple-negative breast
cancer cell line (MDA-MB-231) expressing the green fluorescent protein
(GFP) was monitored by fluorescence microscopy (Fig. 7 and videos SI).
After 1-hour treatment in the presence of nanoreactors, the morphology
of cells changed drastically, maybe due to oxidative stress. After 2
hours, bubbles are produced inside the cells suggesting a high
production of hydrogen peroxide, and the fluorescence starts to
diminish. The bubble production is increasing, and the fluorescence
continues to diminish after 3 hours. Finally, after 4 hours the cells
are very damaged, and the fluorescence has completely disappeared.