2.8 Fluorescence microscopy
Cell culture of 5x105 of MDA-MB231/eGPF (Cell Biolabs
Inc, Ref. AKR-201) was seeded onto cell culture-treated Petri dishes (3
cm of diameter) and incubated in DMEM cultute medium supplement with
10% foetal bovine serum (Biowest, France) and 1% of
antibiotic/antimycotic (GIBGO) overnight at 37°C under 5%
CO2 atmosphere. Afterward, cells were washed with
phosphate buffer saline (PBS) 1X. Then, 100 µg of VLP-GOx resuspended in
DMEM were added to the cell culture and incubated. Time-lapse images of
cells were obtained by LS720 Microscope Lumascope (Etaluma Inc., San
Diego, CA) every 2 minutes for 4 hours. Phase contrast and green
fluorescent (green filter 473-491/502-561 excitation/emission) images
were obtained.