\documentclass[10pt]{article}

\usepackage{fullpage}
\usepackage{CJKutf8} 
\usepackage[utf8]{inputenc}
\usepackage{setspace}
\usepackage{parskip}
\usepackage{titlesec}
\usepackage[section]{placeins}
\usepackage{xcolor}
\usepackage{breakcites}
\usepackage{lineno}
\usepackage{hyphenat}





\PassOptionsToPackage{hyphens}{url}
\usepackage[colorlinks = true,
            linkcolor = blue,
            urlcolor  = blue,
            citecolor = blue,
            anchorcolor = blue]{hyperref}
\usepackage{etoolbox}
\makeatletter
\patchcmd\@combinedblfloats{\box\@outputbox}{\unvbox\@outputbox}{}{%
  \errmessage{\noexpand\@combinedblfloats could not be patched}%
}%
\makeatother


\usepackage{natbib}




\renewenvironment{abstract}
  {{\bfseries\noindent{\abstractname}\par\nobreak}\footnotesize}
  {\bigskip}

\titlespacing{\section}{0pt}{*3}{*1}
\titlespacing{\subsection}{0pt}{*2}{*0.5}
\titlespacing{\subsubsection}{0pt}{*1.5}{0pt}


\usepackage{authblk}


\usepackage{graphicx}
\usepackage[space]{grffile}
\usepackage{latexsym}
\usepackage{textcomp}
\usepackage{longtable}
\usepackage{tabulary}
\usepackage{booktabs,array,multirow}
\usepackage{amsfonts,amsmath,amssymb}
\providecommand\citet{\cite}
\providecommand\citep{\cite}
\providecommand\citealt{\cite}
% You can conditionalize code for latexml or normal latex using this.
\newif\iflatexml\latexmlfalse
\providecommand{\tightlist}{\setlength{\itemsep}{0pt}\setlength{\parskip}{0pt}}%

\AtBeginDocument{\DeclareGraphicsExtensions{.pdf,.PDF,.eps,.EPS,.png,.PNG,.tif,.TIF,.jpg,.JPG,.jpeg,.JPEG}}

\usepackage[utf8]{inputenc}
\usepackage[greek,ngerman,english]{babel}



\usepackage{float}








\begin{document}
\begin{CJK}{UTF8}{gbsn}

\title{Zero order and area under curve spectrophotometric methods for
determination of ampicillin trihydrate in pharmaceutical formulation}



\author[1]{Digambar  MA}%
\affil[1]{Affiliation not available}%


\vspace{-1em}



  
  \date{May 08, 2026}


\begingroup
\let\center\flushleft
\let\endcenter\endflushleft
\maketitle
\endgroup





\selectlanguage{english}
\begin{abstract}
Simple, fast and reliable spectrophotometric methods were developed for
determination of Ampicillin Trihydrate in bulk and pharmaceutical dosage
forms. The solutions of standard and the sample were prepared in double
Methanol. The quantitative determination of the drug was carried out
using the zero order derivative values measured at 205 nm and the area
under the curve method values measured at 202-207 nm. Calibration graphs
constructed at their wavelengths of determination were linear in the
concentration range of Ampicillin Trihydrate using 5-25 \selectlanguage{greek}μ\selectlanguage{english}g/ml (r\selectlanguage{ngerman}²=0.999
and r²=0.999) for zero order and area under the curve spectrophotometric
method. All the proposed methods have been extensively validated as per
ICH guidelines. There was no significant difference between the
performance of the proposed methods regarding the mean values and
standard deviations. Developed spectrophotometric methods in this study
are simple, accurate, precise and sensitive to assay of Ampicillin
Trihydrate in tablets.%
\end{abstract}\selectlanguage{ngerman}%



\sloppy




\selectlanguage{english}
\FloatBarrier
\end{CJK}\end{document}

