Thermal conductivity of soil is one of the phenomenon which measure the amount of heat passing per cross-sectional area. Thermal conductivity of soils depends on various factors. In this study the influence of presence of Microorganisms on enhancement of thermal conductivity of soils is determined experimentally. In this research, soil samples from four different regions namely Dholera, Navsari, Sabarmati River and Madhavpur of Gujarat, India, were taken. The aim of study was to develop experimental model to measure the enhancement of thermal conductivity of soil by microorganisms. The laboratory model which measures thermal conductivity of soil is based on guarded hot plate method. The colonies of microorganisms available in soil were studied by serial dilution and spread plate technique. The influence of microorganisms on soil structure after the treatment was analysed by microscope. Four types of microorganisms were introduced in the soil and the thermal conductivity was recorded after one week of curing period. The microorganisms which gave better result of thermal conductivity were selected for further study. Thereafter, thermal conductivity of sample was measured for curing period of 7, 14, 21 and 28 d. The results of thermal conductivity and soil aggregations were compared after pre and post treatment.