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Genomic diversity of mpox virus in Paris area (France) during the 2022 outbreak
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  • Géraldine Piorkowski,
  • Jade Ghosn,
  • Romain Coppée,
  • Morgane Mailhe,
  • Valentine M. Ferré,
  • Nadhira Houhou-Fidouh,
  • Yazdan Yazdanpanah,
  • Quentin Le Hingrat,
  • Hervé Raoul,
  • Charlotte Charpentier ,
  • Diane Descamps,
  • Xavier de Lamballerie
Géraldine Piorkowski
Aix-Marseille Universite

Corresponding Author:geraldine.piorkowski@inserm.fr

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Jade Ghosn
Hopital Bichat - Claude-Bernard
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Romain Coppée
Hopital Bichat - Claude-Bernard
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Morgane Mailhe
Hopital Bichat - Claude-Bernard
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Valentine M. Ferré
Hopital Bichat - Claude-Bernard
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Nadhira Houhou-Fidouh
Hopital Bichat - Claude-Bernard
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Yazdan Yazdanpanah
Hopital Bichat - Claude-Bernard
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Quentin Le Hingrat
Hopital Bichat - Claude-Bernard
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Hervé Raoul
Agence Nationale de Recherches sur le SIDA et les Hepatites Virales
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Charlotte Charpentier
Hopital Bichat - Claude-Bernard
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Diane Descamps
Hopital Bichat - Claude-Bernard
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Xavier de Lamballerie
Aix-Marseille Universite
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Abstract

In May 2022, several countries reported mpox cases from patients without history of traveling to endemic areas. France was one of the most affected European countries. In this study, we described the clinical characteristics of mpox cases in France, and studied the genetic diversity of the virus. Patients diagnosed with mpox infection (qPCR ct<28) between 21 th May and 4 th July 2022 and between 16 th August and 10 th September 2022 were included to this study. Twelve amplicons corresponding to the most polymorphic regions of the mpox genome and covering ~30,000 nucleotides were generated and sequenced using the S5 XL Ion Torrent technology to evaluate the genetic diversity of mpox sequences. One hundred and fourty-eight patients were diagnosed with mpox-infection. 95% were men, 5% transgender (M-to-F), 50% were taking HIV pre-exposure prophylaxis, and 25% were HIV seropositive. One hundred and sixty-two samples were sequenced and compared to GenBank sequences. Thirty-two distinct mutational patterns were identified. Overall, low genetic diversity of mpox sequences was found compared with pre-epidemic Western-African sequences, with 32 distinct mutational patterns. Our results provide a first glance at the mutational landscape of early mpox 2022 circulating strains in Paris (France).
14 Apr 2023Submitted to Journal of Medical Virology
15 Apr 2023Submission Checks Completed
15 Apr 2023Assigned to Editor
15 Apr 2023Review(s) Completed, Editorial Evaluation Pending
17 Apr 2023Reviewer(s) Assigned
10 May 2023Editorial Decision: Revise Major
26 May 20231st Revision Received
26 May 2023Submission Checks Completed
26 May 2023Assigned to Editor
26 May 2023Review(s) Completed, Editorial Evaluation Pending
26 May 2023Reviewer(s) Assigned
26 May 2023Editorial Decision: Accept