2.4. Preparation of standard solution and electrochemical
measurement of glucose biosensors
Glucose standard solutions with concentrations of 1, 3, 10, 50, and 100
mM were prepared and stored in a refrigerator at 37.5°C before
performing the electrochemical measurement. The RE, CE, and WE were
connected to a potentiostat (WizECM-1200 premium, Wizmac, Korea), and
the cyclic voltammetric (CV) and chronoamperometric (CA) measurements
were performed at room temperature. CV measurements indicated the
oxidation and reduction of glucose and were performed at a scan rate of
100 mV·s−1 for the potential range between −1.0 and
+1.0 V. CA measurements were performed for 20 s at regular intervals of
3 s at a detection potential of +0.6V for various glucose
concentrations, such as 1, 3, 10, 50, and 100 mM.