Figure 7. (A) The graphical data represents the percentage increase in WST-8 reduction through calorimetric measurements performed at 450 nm. The percentage increase in O.D. illustrates the number of viable hepatocytes in the LOC over a period of 10 days. (* p < 0.05) (B) Viability studies on the LOC platform for various concentrations of diclofenac over a period of 48 h.
To determine the toxic sensitivity of our fabricated 3D in vitro model, we performed a drug- toxicity test on the LOC (Fig. 7B). DF is a widely used anti-inflammatory drug. Multiple studies have reported that DF overdose causes liver toxicity and failure. We performed a cellular viability drug-toxicity test to study the dose-dependent drug toxicity on the hepatocytes in the LOC. LOC treatment with 1000 µM of DF exhibited a hepatocyte viability of 20%, indicating the acute toxicity of the drug at this concentration range. At a concentration of 100 µM, the percentage viability increased up to 70%, whereas the viability was more than 90% in the DF concentration below 10 µM. These results demonstrate that the developed LOC model is sensitive and has an improved hepatoxic predication over a wide range of drug concentrations.