Figure 7. (A) The graphical data represents the percentage increase in
WST-8 reduction through calorimetric measurements performed at 450 nm.
The percentage increase in O.D. illustrates the number of viable
hepatocytes in the LOC over a period of 10 days. (* p <
0.05) (B) Viability studies on the LOC platform for various
concentrations of diclofenac over a period of 48 h.
To determine the toxic sensitivity of our fabricated 3D in vitro model,
we performed a drug- toxicity test on the LOC (Fig. 7B). DF is a widely
used anti-inflammatory drug. Multiple studies have reported that DF
overdose causes liver toxicity and failure. We performed a cellular
viability drug-toxicity test to study the dose-dependent drug toxicity
on the hepatocytes in the LOC. LOC treatment with 1000 µM of DF
exhibited a hepatocyte viability of 20%, indicating the acute toxicity
of the drug at this concentration range. At a concentration of 100 µM,
the percentage viability increased up to 70%, whereas the viability was
more than 90% in the DF concentration below 10 µM. These results
demonstrate that the developed LOC model is sensitive and has an
improved hepatoxic predication over a wide range of drug concentrations.