3.6. Biosensor integration of LOC and glucose concentration
measurement after drug test
The designed LOC platform includes two external medium blocks on both
sides of the chip and a central space for holding the 3D-printed liver
in-vitro model. The external medium blocks serve two purposes: (1) it
allows the free movement of the medium through the hollow lumen of the
3D model, simulating a perfusion-based liver in-vitro model, and (2) it
provides space for the easy placement and removal of biosensors onto the
LOC. In OOC development, chip and sensor integration is a crucial task
for eliminating various complex intermediate processes, such as enabling
real-time cell monitoring. Extensive research has been conducted on OOC
and sensor integration in recent years; however, few studies have used
CP as a sensor for integration with the chip. Therefore, in this study,
we have integrated 3D printed glucose biosensor and bioprinted
liver-on-a-chip to determine the accuracy and sensitivity of the
fabricated CP-based electrode coated with Nafion/MWCNT/GOx.