Agarose Gel
1 % agarose gel was made with 1 g UltraPure Agarose (Invitrogen), 100 mL 0.5X TBE Buffer diluted from TBE Buffer 10X (Biosolve) and 10 µL Midori Green Advance (Nippon Genetics). 8 µL of each sample was mixed with 2 µL DNA Gel Loading Dye 6X (Thermo Scientific) and 10 µL of 100 bp DNA Ladder Ready to Load size marker (Solis Biodyne) was used as reference. Migration took 45 min at 120 V with Owl EC300XL Compact Power Supply (Thermo Scientific). Gel visualization and analysis were done using Alliance Q9 Advanced imaging system and software (Uvitec Cambridge).