DNA Extraction
DNA was extracted from midguts, carcasses or legs. DNA extraction was performed with ZymoBiomics 96 MagBead DNA Kit (Zymo Research) for midguts and with HighPrep Blood & Tissue DNA Kit (MagBio) for carcasses and legs. Samples were mixed using 0.5 mm glass beads in 2 mL screwcap tubes with a Precellys Evolution (Bertin Technologies) bead beater homogenizer. Automatic DNA extraction was performed with the KingFisher Duo Prime system (Thermo Scientific). DNA was eluted in ZymoBiomics DNAse/RNAse Free Water from the kit for midguts and MB Elution Buffer supplied in the kit for carcasses and legs. Extracted DNA was kept at -80 °C until further utilization.