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Expanding the synthetic biology repertoire of a fast-growing cyanobacterium Synechococcus elongatus PCC 11801
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  • Pramod Wangikar,
  • Swati Madhu,
  • Annesha Sengupta,
  • ADITYA SARNAIK
Pramod Wangikar
Indian Institute of Technology Bombay Department of Chemical Engineering

Corresponding Author:wangikar@iitb.ac.in

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Swati Madhu
Indian Institute of Technology Bombay Department of Chemical Engineering
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Annesha Sengupta
Indian Institute of Technology Bombay Department of Chemical Engineering
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ADITYA SARNAIK
Indian Institute of Technology Bombay Department of Chemical Engineering
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Abstract

Synechococcus elongatus PCC 11801 is a fast-growing cyanobacterium, exhibiting high tolerance to environmental stresses. We have earlier characterized its genome and analysed its transcriptome and proteome. However, to deploy it as a potential cell factory, it is necessary to expand its synthetic biology toolbox, including promoter elements and ribosome binding sites (RBSs). Here, based on the global transcriptome analysis, 48 native promoters of the genes with high transcript count were characterized using a fluorescent reporter system. P cpcB, P psbA1, P 11770 promoters exhibited consistently higher fluorescence under all the cultivation conditions. Similarly, from the genome data and proteome analysis, 534 operons were identified. Fifteen intergenic regions exhibiting higher protein expression from the downstream gene were systematically characterized for RBSs, using an operon construct comprising of fluorescent protein genes eyfp and mTurq under P cpcB (P cpcB: eyfp:RBS: mTurq:T rrnB). Overall, the work presents promoter and RBS sequence libraries, with varying strengths, to expedite bioengineering of PCC 11801.
Submitted to Biotechnology and Bioengineering
09 Mar 2024Review(s) Completed, Editorial Evaluation Pending
06 May 2024Reviewer(s) Assigned
08 May 2024Editorial Decision: Accept