Figure Legend:
Figure 1. Flow cytometry and FISH results of T/My mixed
phenotype acute leukemia. A: The peripheral blood flow cytometry
identified a major blast population expressing equivocal cytoplasmic CD3
(with intensity not reaching to the CD3 intensity of normal T cells),
and a small population of MPO+ CD34+ blasts (less than 5%). B: The
repeated flow cytometry on bone marrow aspirate revealed cytoplasmic CD3
dim+ (with intensity equal to normal T cells) and cytoplasmic MPO in a
sub-population of blasts (5-10%). C: Representative blast on bone
marrow aspirate (originally 1000x Gimsa stain). D: The FISH study
revealed a copy gain of BCL11B in 58 out of 100 cells (green for
BCL11B probe; red for TLX3 probe.). CD3 (E, 200x) and MPO (F, 200X)
immunostains are positive on a subset of blasts on core biopsy, which
also support the diagnosis of T/My MPAL.