Remarkable efforts have been made for room-temperature phosphorescence (RTP) materials, however, achieving desirable RTP materials in a simple and efficient way has always been the focus of researchers. Here, we reported a high-performance RTP platform based on hydrogen-bonded organic frameworks (HOFs), by simply and efficiently in-situ encapsulating phosphor into HOFs materials, the obtained host guest materials based on HOFs not only exhibited a phosphorescent lifetime of up to 4.91 ms, but also resisted water and oxygen quenching agents to maintain phosphorescence in aqueous solution. Besides, attractive triplet-to-singlet Förster resonance energy transfer (TS-FRET) platform could be easily fabricated by embedding acceptor into the HOFs-based host guest materials. Furthermore, the proposed HOFs-based RTP materials could be facilely processed into luminescent film. Such HOFs-based RTP strategy are expected to act as a facile and versatile strategy to prepare outstanding RTP materials.