Influence of specific ECM proteins on liver-specific functions
After confirming successful covalent ECM tethering to MPS, we moved to
assess biological responses in spheroids by combining hepatocytes and
stellate cells with modified MPs (Figure 5A) , specifically to
study potential impact of different ECM proteins on liver-specific
functions. In culture, viable hepatocytes synthesize and excrete albumin
and urea into the surrounding medium, replicating important functions
they perform in vivo in the liver. To characterize these critical
cellular functions, we collected culture medium and measured albumin and
urea production at specific time points.