Figure 7: Cell heterogeneity in spheroids using HepG2: HSC
(4:1), 20k cells initially per spheroid cultured with different
oxygenating MPs. A) Representative confocal microscopic images of
central plane of spheroids stained with CYP3A4 (for HepG2 cells, green),
GFAP (for HSC cells, purple), and Hoechst 33342 (for nuclei, blue).
Arrows highlight the localization of HSCs. B) Quantitative analysis of
images using ImageJ. Statistics via one-way ANOVA and Tukey’s post
hoc testing with A as the highest mean and C with the lowest mean,
p< 0.05. Mean ± SD, n =2 with S=3.