Figure 4: EDC/NHS coupling reaction for bioconjugation of ECM proteins to MPs. A) Schematic of EDC/NHS reaction between protein carboxyl residues and free amine groups from PFC- oxygenating MPs in varied pHs. B) Quantification of covalently tethered protein (laminin 111) via BCA assay kit. Data represented as mean ± SD, n = 4. Statistical analysis via one-way ANOVA with Tukey’s post hoctesting with A as the highest mean and C with the lowest mean. C) Atomic composition of modified and non-modified PFC- MPs measured via XPS. The N1S percentage correlates to the concentration of proteins attached to the surface.