not-yet-known not-yet-known not-yet-known unknown The proteomic analysis of clinical samples using mass spectrometry (MS) is a modern approach for elucidating pathogenesis and molecular profiling. We applied a state-of-the-art proteome analysis technology that can detect >2,000 proteins from serum samples in single-shot proteome analysis by combining a high-abundance protein depletion method using antibody columns and highly advanced data-independent acquisition (DIA)-MS technology10. Saliva analysis also holds promise because it enables the evaluation of more proteins than serum and is even less invasive.