50×106 MSC(M) from 3 donors were expanded on
CellStart-coated plastic microcarriers with S/X-free StemPro culture
medium. In the end of the EVs conditioning phase (last 2 days), at days
9 and 13, the MSC-CM from FB and FB/CP cultures, respectively, were
separated from the cell-containing microcarriers, streamed through a
0.22 µm filter and stored at -80ºC for EVs isolation and
characterization. Results are presented as mean ± SEM. MSC-EV:
mesenchymal stromal cell-derived extracellular vesicles, MSC-CM:
mesenchymal stromal cell-derived conditioned medium, PPR: particle to
protein ratio, FB: fed-batch, FB combined with continuous perfusion:
FB/CP, STR: stirred tank reactor, FI: Fold increase in total cell
number. |
50×106 MSC(M) from 3 donors were expanded on
CellStart-coated plastic microcarriers with S/X-free StemPro culture
medium. In the end of the EVs conditioning phase (last 2 days), at days
9 and 13, the MSC-CM from FB and FB/CP cultures, respectively, were
separated from the cell-containing microcarriers, streamed through a
0.22 µm filter and stored at -80ºC for EVs isolation and
characterization. Results are presented as mean ± SEM. MSC-EV:
mesenchymal stromal cell-derived extracellular vesicles, MSC-CM:
mesenchymal stromal cell-derived conditioned medium, PPR: particle to
protein ratio, FB: fed-batch, FB combined with continuous perfusion:
FB/CP, STR: stirred tank reactor, FI: Fold increase in total cell
number. |
50×106 MSC(M) from 3 donors were expanded on
CellStart-coated plastic microcarriers with S/X-free StemPro culture
medium. In the end of the EVs conditioning phase (last 2 days), at days
9 and 13, the MSC-CM from FB and FB/CP cultures, respectively, were
separated from the cell-containing microcarriers, streamed through a
0.22 µm filter and stored at -80ºC for EVs isolation and
characterization. Results are presented as mean ± SEM. MSC-EV:
mesenchymal stromal cell-derived extracellular vesicles, MSC-CM:
mesenchymal stromal cell-derived conditioned medium, PPR: particle to
protein ratio, FB: fed-batch, FB combined with continuous perfusion:
FB/CP, STR: stirred tank reactor, FI: Fold increase in total cell
number. |
50×106 MSC(M) from 3 donors were expanded on
CellStart-coated plastic microcarriers with S/X-free StemPro culture
medium. In the end of the EVs conditioning phase (last 2 days), at days
9 and 13, the MSC-CM from FB and FB/CP cultures, respectively, were
separated from the cell-containing microcarriers, streamed through a
0.22 µm filter and stored at -80ºC for EVs isolation and
characterization. Results are presented as mean ± SEM. MSC-EV:
mesenchymal stromal cell-derived extracellular vesicles, MSC-CM:
mesenchymal stromal cell-derived conditioned medium, PPR: particle to
protein ratio, FB: fed-batch, FB combined with continuous perfusion:
FB/CP, STR: stirred tank reactor, FI: Fold increase in total cell
number. |