This research showed that the iron nanoparticles (FeNPs) formulated with Ocimum basilicum aqueous extract having potent antioxidant and anti-human glioma activities in vitro condition. For determinate the properties of the FeNPs that were produced from the reaction between iron chloride solution with aqueous Ocimum basilicum extract, we used UV–Visible Spectroscopy (UV-Vis), Field Emission Scanning Electron Microscopy (FE‐SEM), and Fourier Transformed Infrared Spectroscopy (FT‐IR). To survey the cytotoxicity and anti-human glioma effects of FeNPs, MTT assay was used on the SW1088, LN-229, U-87 and A-172 cell lines. For investigating the antioxidant properties of FeNPs, the DPPH test was used in the presence of butylated hydroxytoluene as the positive control. Iron nanoparticles had very low cell viability and anti-human glioma properties dose-dependently against SW1088, LN-229, U-87 and A-172 cell lines without any cytotoxicity on the normal cell line. The IC50 of the iron nanoparticles were 101, 75, 97, and 54 µg/ml against SW1088, LN-229, U-87 and A-172 cell lines. DPPH test revealed similar antioxidant potentials for FeNPs and butylated hydroxytoluene. It seems that the anti-human glioma effects of recent nanoparticles is due to their antioxidant properties.

Nanomedicine is a way for the treatment of the several types of cancers. In the present study, gold nanoparticles were green-synthesized using the aqueous extract of Mangifera indica. The chemical characterization was studied different techniques including FT-IR, UV-Vis, SEM and TEM. The anti-human gastric cancer activity of AuNPs@Mangifera indica was evaluated using MTT assay. The nanoparticles were formed in a spherical shape with a size less than 100 nm for the particle size. The treated cells with AuNPs@Mangifera indica were assessed by MTT assay about the cytotoxicity and anti-human gastric cancer properties on normal (HUVEC) and gastric cancer cell lines i.e. GC1415, GC1436, NCI-N87 and MKN45. In the antioxidant test, the IC50 of AuNPs@Mangifera indica and BHT against DPPH free radicals were 55 and 49 µg/mL, respectively. The viability of malignant gastric cell lines reduced dose-dependently in the presence of AuNPs@Mangifera indica. The IC50 of AuNPs@Mangifera indica were 212, 213, 250 and 328 µg/mL against GC1415, GC1436, NCI-N87 and MKN45 cell lines, respectively.