Experimental study of the phase and amplitude observations of sub-ionospheric very low and low frequency signals is performed to analyze the response of the lower ionosphere during the August 21, 2017 total solar eclipse in the United States of America. Three subionospheric wave paths have been investigated. The length of the paths varied from 2200 to 6500 km, signal frequencies were 21.4 kHz, 25.2 kHz and 40.8 kHz. Two paths crossed the region of total eclipse and the third path was in the region of 40-60% of obscuration. None of the signals revealed any noticeable amplitude changes during the eclipse while negative phase anomalies (from -35° to -95°) were detected for all three paths. It was shown that the effective reflection height of the ionosphere in low and middle latitudes has been increased by 3.5-5 km during the eclipse.