Low temperature (LT)-induced membrane lipid metabolism resulted in different degrees of chilling injury (CI) in five different chill-sensitive peach varieties. Among them, ‘Chibaifen’ peaches had the most serious occurrence of CI and the strongest cold sensitivity by having the largest up- and down-regulated numbers of phosphatidylcholine (PC) and triacylglycerol (TAG) components and maintaining higher levels of fold change, peak area abundance and unsaturation of TAG components induced by LT. However, ‘Hujing’ peaches had the most lightest occurrence of CI and had the strongest cold tolerance by having the more up-regulated numbers of PC and TAG components, but maintaining lower levels of fold change, peak area abundance and unsaturation of each lipid component induced by LT. This study provides suitable research materials for improving chill-sensitive peach varieties and developing effective fresh-keeping technologies and a scientific basis for improving the quality competitiveness of cold-tolerant peach varieties.