The dissipative particle dynamics (DPD) method is used to explore the aggregated distribution state and the interaction energy of Natural Deep Eutectic Solvent (NADES) (lactic acid/glucose/H2O)/Tween-20/methanol/ cyclohexane microemulsion system. The phase transformation point of the system is determined by combining the change of IFT value and aggregated distribution state, which is used to draw the simulated phase diagram of the microemulsion system. The results show as follows: Surfactants have a restrictive effect on the distribution of microemulsion components, and the increase in the proportion of surfactants gradually restricts the molecular movement of cyclohexane. When the surfactant mass fraction reaches the threshold of 56.25%, the properties of the system change qualitatively, and the cyclohexane/NADES microemulsion is formed. The interaction energy of methanol-Head group and cyclohexane-Tail group is the main driving force for the formation of the microemulsion system.

The negative impact of the synthetic antimicrobials and the overuse of antibiotics have caused global concern, which necessitates the development of a new form of antimicrobials that are derived from natural compounds. In this study, the synthetic route of producing water soluble iso-fatty acid arginate hydrochloride derivatives is investigated by introducing branched (iso-) chain and other linear- (n-) chain fatty acids to the amino acid “arginine” molecules. The two-step synthesis is straightforward and provides an efficient 91%, 88%, and 76% product yield for ethyl n-lauroyl arginate hydrochloride, ethyl n-oleoyl arginate hydrochloride, and ethyl iso-oleoyl arginate hydrochloride, respectively. ATR-FT-IR, NMR, and LC-MS-Q-TOF techniques are used to thoroughly characterize the products. The antimicrobial characteristic of the synthesized ethyl n-lauroyl arginate (LAE) shows a similar activity against both Gram+ and Gram- bacteria as the commercial LAE product. However, its analogs are found to have antimicrobial property primarily against Gram-positive bacteria.