4 Conclusions
In this study, we reported the complete mitogenomes of twoProphantis species, P. octoguttalis and P. adusta , belonging to the tribe Trichaeini, for the first time, and analyzed their gene size and arrangement, base composition, codon usage, and tRNA secondary structure, etc., which were highly consistent with those of other previously studied species of Spilomelinae. The two mitogenomes were typical of lepidopteran insects. Combined with the published mitogenome sequences of Crambidae, all phylogenetic trees based on the different datasets confirmed the monophyly and position of Trichaeini and showed satisfactorily high support values. However, its sister group was not completely resolved, combined with previous multisite studies. In addition, the phylogenetic relationships within Crambidae in phylogenetic tree in our present study were in general agreement with previous studies, whereas the affinities in the “non-PS clade” were still unstable and require further investigation. Therefore, improving sample coverage and combining different molecular markers, such as mitochondrial genome and nuclear genes, should be considered in the future research on these taxa.