Previous studies have suggested variability in soybean oil quality depending on geographic origin of the soybeans. In this study, 1,035 crude degummed soybean oil samples collected from vessels and crushers over five harvest years (2020–2024) were analyzed for 11 key quality attributes. Soybean oil samples were sourced from soybeans that originated from the United States, Brazil, and Argentina. The results indicate that U.S.-grown soybeans consistently produce the highest-quality oil, characterized by the lowest free fatty acid (FFA) levels and lowest neutral oil loss (NOL). Oils from U.S. soybeans also exhibited the highest iodine values, likely reflecting cooler growing temperatures. In contrast, Argentine oil quality was intermediate, showing moderate FFA and NOL levels, while Brazilian soybean oil displayed the lowest overall quality, with elevated FFA, NOL, and mineral content (calcium, magnesium, phosphorus). These differences may stem from greater moisture exposure or heat damage during harvest and storage in tropical regions. Differences in unsaponifiable matter, saponification value, iron content, and Lovibond red color were minimal or inconsistent across origins. The chlorophyll content varied primarily by year and environmental conditions rather than geographic source. Collectively, these findings demonstrate that soybean oil quality differs significantly among growing regions, with potential economic implications for processors and traders.