Determination of root and shoot lengths after sucrose, liquid
medium, and culture stroma treatments
The SRPs of 1539-1 were transferred to the growth medium.Sucrose treatment : the growth medium was a 1/2 WPM liquid
medium (30 mL), which was supplemented with culture stroma vermiculite:
perlite =1:1 (1/5 volume of flask, approximately) and sucrose (10, 20,
30, and 40 g/L). Each SRP was transferred into a dedicated flask, and
each group was treated with 15 plantlets and repeated three times. After
one-month of culturing under cool white fluorescent light (16/8h) at
23±1℃, the regenerated plantlets were removed from the flasks and the
roots were rinsed. Next, the roots and root shoot lengths (total lengths
of the aboveground parts) of the regenerated plants were measured with
the scale.
Liquid medium treatment: the growth medium was supplemented
with sucrose (20 g/L), culture stroma vermiculite: perlite =1:1
(~1/5 volume of flask) and the liquid medium (DCR, LP,
GD, and 1/2WPM) (30 mL). The other conditions were the same as the
sucrose treatments.
Transplantation stroma treatment: The growth medium contained
30 mL of a 1/2WPM liquid medium, which was supplemented with 20 g/L
sucrose, and a 1/5 flask volume of transplantation stroma (perlite,
vermiculite, perlite and vermiculite = 1:1, respectively). The other
conditions were identical to sucrose treatments. The transplantation
stroma was bottled for sterilization and cooled prior to adding the
sterilized liquid medium. Following growth enhancement, the plantlets
were transplanted into a field for domestication.