Conclusion
For this study, we initially articulated the acclimation protocols for the somatic plantlets (SRPs) of nematode-resistant P. thunbergii , and monitored their survival rates following transplantation. The results indicated that a growth medium containing 20 g/L sucrose, 1/2 WPM liquid medium, and a transplantation stroma (vermiculite and perlite = 1:1) was optimal for improving the P. thunbergii plantlet quality. Moreover, blue light promoted the shoot length, whereas red light promoted the main root length. The survival rates of regenerated plants were improved by all treatments that enhanced the SRP growth. This study investigated the factors involved in the transition from the heterotrophic (organic nutrient growth) to autotrophic (inorganic nutrient growth) stage of SRPs. The culture conditions were identified to improve the quality and the survival rates of the SRPs that were transplanted into the field. This research provides the foundation for future research toward increasing the survival rates of plantlets, and promotes the application of elite clones (nematode-resistant P. thunbergii plantlets)