Determination of root and shoot length following brassinolide
treatments
The SRPs were obtained from 1637-2 cell lines, and the SRPs with shoots
but no radicles were transferred to the rooting medium. The rooting
medium was supplemented with 1/4 WPM (large element reduction),
brassinolide (0, 10, and 1 µg/L), 20 g/L sucrose, 0.5g/L activated
carbon, and 6 g/L agar. Each SRP was transferred into a dedicated flask.
Each group was treated with 15 plants, which was repeated three times.
The plantlets were cultured on the rooting medium for one-month under
cool white fluorescent light (16/8h) at 25±1℃. The plantlets were then
removed from the rooting medium and the roots were rinsed, after which
the roots and shoot lengths (total lengths of the aboveground parts)
were measured with a scale.