Conclusion
For this study, we initially articulated the acclimation protocols for
the somatic plantlets (SRPs) of nematode-resistant P. thunbergii ,
and monitored their survival rates following transplantation. The
results indicated that a growth medium containing 20 g/L sucrose, 1/2
WPM liquid medium, and a transplantation stroma (vermiculite and perlite
= 1:1) was optimal for improving the P. thunbergii plantlet
quality. Moreover, blue light promoted the shoot length, whereas red
light promoted the main root length. The survival rates of regenerated
plants were improved by all treatments that enhanced the SRP growth.
This study investigated the factors involved in the transition from the
heterotrophic (organic nutrient growth) to autotrophic (inorganic
nutrient growth) stage of SRPs. The culture conditions were identified
to improve the quality and the survival rates of the SRPs that were
transplanted into the field. This research provides the foundation for
future research toward increasing the survival rates of plantlets, and
promotes the application of elite clones (nematode-resistant P.
thunbergii plantlets)