Determination of root and shoot lengths after red and blue LED light treatments
Experiments that investigated the impacts of different light wavelengths on SRP growth were conducted with the 1637-2 cell lines. The SRP was transferred to the growth medium which was supplemented with 30 mL of a 1/2WPM liquid medium, 20 g/L sucrose, and 1/5 flask volume of culture stroma (perlite and vermiculite = 1:1, respectively). Each SRP was transferred into a dedicated flask. The plantlets were cultured for one-month under LED treatments (16/8h) at 25±1℃c. LEDs with different red and blue ratios were used as the light sources (B: blue light; 5R5B: red light: blue light =5:5; 7R3B: red light: blue light =7:3; 8R2B: red light: blue light =8:2, R: red light). The cool white fluorescent lamp served as the CK. Each group was treated with 10 plantlets and repeated three times. The root growth parameters were measured using a root scanner, and the shoot lengths were measured with a scale.