Determination of root and shoot lengths after sucrose, liquid medium, and culture stroma treatments
The SRPs of 1539-1 were transferred to the growth medium.Sucrose treatment : the growth medium was a 1/2 WPM liquid medium (30 mL), which was supplemented with culture stroma vermiculite: perlite =1:1 (1/5 volume of flask, approximately) and sucrose (10, 20, 30, and 40 g/L). Each SRP was transferred into a dedicated flask, and each group was treated with 15 plantlets and repeated three times. After one-month of culturing under cool white fluorescent light (16/8h) at 23±1℃, the regenerated plantlets were removed from the flasks and the roots were rinsed. Next, the roots and root shoot lengths (total lengths of the aboveground parts) of the regenerated plants were measured with the scale.
Liquid medium treatment: the growth medium was supplemented with sucrose (20 g/L), culture stroma vermiculite: perlite =1:1 (~1/5 volume of flask) and the liquid medium (DCR, LP, GD, and 1/2WPM) (30 mL). The other conditions were the same as the sucrose treatments.
Transplantation stroma treatment: The growth medium contained 30 mL of a 1/2WPM liquid medium, which was supplemented with 20 g/L sucrose, and a 1/5 flask volume of transplantation stroma (perlite, vermiculite, perlite and vermiculite = 1:1, respectively). The other conditions were identical to sucrose treatments. The transplantation stroma was bottled for sterilization and cooled prior to adding the sterilized liquid medium. Following growth enhancement, the plantlets were transplanted into a field for domestication.