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Promoting the application of pinus thunbergii Parl. toward enhancing the growth and survival rates of post-germination somatic plantlets
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  • Tingyu Sun,
  • Yanli Wang,
  • Xiaoqin Wu,
  • Jianren Ye
Tingyu Sun
Nanjing Forestry University

Corresponding Author:suntingyunjfu@163.com

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Yanli Wang
Anhui Agricultural University
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Xiaoqin Wu
Nanjing Forestry University
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Jianren Ye
Nanjing Forestry University
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Abstract

There is a growing need for nematode-resistant Pinaceae species seedlings to cope with the degradation of global coniferous forests due to the prevalence of pine wilt disease. One of obstacle of Pinaceae species plantlets commercialization is the regeneration plantlets from a controlled, sterile environment to the field while maintaining a high survival rate. The low survival rate of plantlets transferred from a lab to soil is mainly because of the quality of plantlet which makes commercialization challenging. For this paper, we investigated the factors that influence the growth of post-germination somatic regeneration plants (SRPs) to promote the application of nematode-resistant of Pinus thunbergii. The results indicated that, for rooted plantlet, a suitable liquid medium (1/2 WPM), transplantation stroma (perlite and vermiculite =1:1), and carbohydrate (20 g/L sucrose) were effective for promoting the growth of SRPs. While, for unrooted plantlets, 1 ug/L of brassinolide enhanced plantlets growth and rooting. In the laboratory domestication stage, under light spectrum treatments, blue light (B) significantly promoted the longitudinal growth of shoots, while red light (R) was of benefit for the improvement of root growth. The optimal combination of R/B was the 8:2. After this domestication culture, SRPs of Pinus thunbergii could be directly transplanted to the field with a higher survival rate (85.2%). This is the first report on domestication of conifer regeneration plants, that will increase the possibility of afforestation of Pinaceae or other woody plants. In addition, it contributes to breeding project of pine resistance to pine wilt disease.