Tryptase quantification in cell free supernatant
Culture supernatant from PCIS passively sensitized and stimulated with PE (1µg/ml) or controls was collected 10min post-stimulation and stored at -80oC. Tryptase levels in supernatant were measured using a tryptase beta-2 ELISA (MilliporeSigma, Burlington MA, USA) after concentration using centrifugal filter devices (Amicon Ultra-0.5 10K device, MilliporeSigma, Burlington MA, USA).