scNucSeq of gut tissue confirms the presence of mast cells and other cell types
The cellular composition of PCIS was analyzed using scNucSeq. Single cell gene expression profiles from colon tissue donors (n=4) were compiled to reveal the cellular composition of PCIS (Figure 1D). Expression profiles aligned with the cellular identities of each cluster and were confirmed using single cell gene expression databases.25,26 All expected cell types in colon tissue were observed (Figure 1D, Table S4) including epithelial cells (enterocytes, goblet cells, enteroendocrine cells, endothelial cells), stromal cells (fibroblasts, smooth muscle cells) and immune cells (T cells, γδT cells, B cells, mast cells, dendritic cells, macrophages).