PCIS maintain viability for at least 24h
This model was generated to study acute, IgE-mediated allergic reactions, which are known to occur within seconds of exposure to a relevant allergen. To confirm that PCIS remained viable during the incubation process, they were monitored from 0-24h post-slicing. Cell death (LDH) and metabolism (WST-1) were assessed in each sample and demonstrated consistent viability of PCIS during the investigated 24h in culture (Figure 1B, C).