Video microscopy
PCIS muscle contraction was filmed using a stereomicroscope with a camera attachment (Walter Products, ON, Canada; TCapture Software, Tucsen Photonics, Fuzhou, China) for 10min without the addition of stimuli to establish baseline movement of the individual sample. The slices were then filmed for an additional 10min upon addition of either peanut extract (PE) (1µg/ml, ALK-Abelló, Hørsholm, Denmark), serotonin (10µg/ml, MilliporeSigma, Darmstadt, Germany), FcɛRI-crosslinker (10µl, Bühlmann, Amherst, NH, USA), histamine (10µg/ml, ALK-Abelló, Hørsholm, Denmark) or ovalbumin (OVA; 1µg/ml, InvivoGen, San Diego, CA, USA) as an irrelevant allergen. Any samples not passing quality control criteria (Table S3) were excluded from analysis.