PCIS maintain viability for at least 24h
This model was generated to study
acute, IgE-mediated allergic reactions, which are known to occur within
seconds of exposure to a relevant allergen. To confirm that PCIS
remained viable during the incubation process, they were monitored from
0-24h post-slicing. Cell death (LDH) and metabolism (WST-1) were
assessed in each sample and demonstrated consistent viability of PCIS
during the investigated 24h in culture (Figure 1B, C).