Figure Legends:
Figure 1 . Phosphorylation of pERK 1/2 (pT202/pY204),
p38 (pT180/pY182), Akt (s473) and mTOR in basophils of allergic (n=6)
and non-allergic individuals (n=3) upon stimulation with peanut
allergen, PMA/Iono, or an anti-FcεRI was assessed via mass cytometry.(A) A histogram illustration of the median changes in protein
phosphorylation in basophils of allergic (n=6) and non-allergic patients
(n=3). (B) Line graph illustrating median changes in protein
phosphorylation in basophils of allergic (n=6) and non-allergic patients
(n=3) after 3 or 15min post-stimulation with peanut extract (0.01-100
ng/ml) expressed as mean ± SEM.
Figure 2. Basophil subset clustering by stimulation and
marker expression. (A) Basophils pooled from peanut-allergic patients
(n=10) clustered with Leiden algorithm. (B) Basophil proportions
stimulated by PMA/Iono, anti-FcεRI, peanut extract, and unstimulated in
subsets 0-6. (C) Clustered basophils from peanut-allergic patients
stimulated with PMA/Iono, anti-FcεRI, 10 ng/ml peanut extract, and
unstimulated. Cells separated by duration of stimulation (0, 3, 15min).
(D) Proportion of 3 and 15min stimulated basophils per subset. (E)
Pseudo-time lineage plot of basophils from peanut-allergic individuals.
Basophils transition from subsets 0 and 1 to subsets 2 and 4. (F) Subset
expression of pERK, mTOR and p38.