Introduction: Laparoscopic lateral suspension (LLS) with mesh is indicated for the treatment of apical prolapse. However, clinical data of this technique still remain limited. Objective: To evaluate the clinical outcomes and changes in the vaginal axis of LLS with mesh for apical and anterior prolapse. Design: Retrospective single center clinical study. Setting: Conducted at the department of Obstetrics and Gynecology, West China Second University Hospital, China. Sample: Ninety-seven patients underwent LLS between 2020 and 2024. Methods: Retrospective analysis. Main Outcome Measures: Anatomical success rate, subjective cure rate, complications rate and changes in the vaginal axis before and after LLS by magnetic resonance imaging (MRI). Results: The anatomical success rate was 98.97% for apical prolapse and 85.57% for anterior prolapse, with a perioperative complication rate of 3.09% in the two-year follow-up. Mesh exposure at the vaginal cuff was observed in five patients (5.15%), all of whom had a concomitant hysterectomy. The patient satisfactory rate (evaluated by PGI-I score ≤3) was 96.67% at 24 months postoperatively. On MRI, no significant differences were observed between the Post-LLS and nulliparous women regarding the angle between the lower and upper vagina or the Cx/Apical line at rest (p> 0.05). Conclusion: LLS is an effective and safe surgical technique for treatment of apical prolapse with or without anterior prolapse, demonstrating low complication rates and high anatomic curative rates in short-term follow-up. MRI examination showed that a level-Ⅰ defect can be effectively addressed well through LLS, highlighting its capability to restore apical support.

Objective: To preliminarily investigate the efficacy and safety of autologous nucleated cell transplantation (ANCT) for treating Vaginal Relaxation Syndrome (VRS) patients. Methods and Samples: Ten married women with vaginal relaxation syndrome were selected from January 2019 to July 2019. All participants were treated via autologous nucleated cell transplantation once a month for three consecutive times, and followed up for 1 month after each treatment. Main Outcome Measures: The degree of vaginal relaxation, the female sexual function index (FSFI), vaginal health index score (VHIS) and subjective symptom improvement scale (PGI-C) were conducted. Results: All the 10 cases successfully completed the ANCT treatment. After ANCT treatment, there were significant improvements in the degree of vaginal relaxation. with the detailed date including (-1.06±0.78, P<0.01) in first time, (-1.30±0.91, P<0.05) in twice time and (-1.5±0.77, P<0.01) three times. Furthermore, the vaginal health index (VHIS) after first treatment (1.63±0.92, P<0.001), twice (4.33±1.51, P<0.001) and three times (3.65±1.77, P< 0.001) were significantly improved. Moreover, the scores of female sexual function index (FSFI) after first treatment (13.50±8.55), twice (18.17 ±6.46) and three times (19.65±6.7) were also substantial variation (P<0.001). According to the subjective symptom improvement score , The study indicates that ANCT can effectively improve the degree of vaginal relaxation, sexual function and vaginal health of patients with VRS, as well as vaginal compactness, wetness, air sensation and vaginal secretions. Conclusion: The novel ANCT method heighten the quality of sexual life may be a better choice for non-surgical treatment of private plastic surgery for VRS patients.