3.2. In silico analysis of Scenedesmaceaecellulases
We have used different bioinformatic tools to analyze the cellulases present in five different species of Scenedesmus:Scenedesmus obliquusEN0004 v1.0, Scenedesmus obliquus UTEX B 3031, Scenedesmusobliquus var. DOE0013 v1.0, Scenedesmus sp . NREL 46B-D3 v1.0, Scenedesmus sp PABB004 and Scenedesmus quadricaudaLWG 002611. The presence of conserved motifs, the arrangement of diverse domains (catalytic domain, carbohydrate-binding module (CBM) and linker regions) and the phylogenetic relationship between the proteins were determined. Their amino acid sequences were compared with enzymes previously characterized from others taxonomic groups.
The cellulases sequences analyzed in this study were classified with KEGG ENZYME Database Entry by Phycocosm 14, into three groups: (i) endo-β-glucanases (EC 3.2.1.4), (ii) β-glucosidases (EC 3.2.1.21) and (iii) cellobiohydrolases or exoglucanases (EC 3.2.1.91) (Table 2).
In S. quadricauda LWG002611 sequences with a higher length than 300 amino acids and with higher similarity with previously studied cellulases are code by the genes: Scequ2611|2009, Scequ2611|9833 and Scequ2611|547 (percentage of identity: 98.32%, 93.93% and 82.26% respectively, table 1), which were chosen to be included in the posterior bioinformatic analysis