Fig. 12A Domain architecture of the putative exoglucanases enzymes in the proteome of Scenedemaceae The figure shows all predicted proteins containing domains annotated as glycosyl hydrolases in families GH10, in the analyzed strains. Red boxes represent signal peptides. Yellow squares represent transmembrane domains
B GH10 family exoglucanase alignment. Multiple alignment between Scenedesmus GH10 exoglucanases, and Celfi|P07986, sequence used as the template for 3D modeling. The multiple sequence alignment was performed using Clustal Omega program and ESPript 3.0. Red asterisk above the alignment indicates catalytic residues. Abbreviations: ScespPA4: Scenedesmus sp. PABB004, Scesp1: Scenedesmus sp. NREL 46B-D3 v1.0, Sceob1:Scenedesmus obliquus UTEX B 3031, SceobDOE: Scenedesmus obliquus var. DOE0013 v1.0, SceobE4: Scenedesmus obliquus EN0004 v1.0, Scequ2611: Scenedesmus quadricauda LWG 002611, Celfi:Cellulomonas fimi .