Fig. 12A Domain architecture of the putative exoglucanases
enzymes in the proteome of Scenedemaceae The figure shows all predicted
proteins containing domains annotated as glycosyl hydrolases in families
GH10, in the analyzed strains. Red boxes represent signal peptides.
Yellow squares represent transmembrane domains
B GH10 family exoglucanase alignment. Multiple
alignment between Scenedesmus GH10 exoglucanases, and
Celfi|P07986, sequence used as the template for 3D modeling.
The multiple sequence alignment was performed using Clustal Omega
program and ESPript 3.0. Red asterisk above the alignment indicates
catalytic residues. Abbreviations: ScespPA4: Scenedesmus sp.
PABB004, Scesp1: Scenedesmus sp. NREL 46B-D3 v1.0, Sceob1:Scenedesmus obliquus UTEX B 3031, SceobDOE: Scenedesmus
obliquus var. DOE0013 v1.0, SceobE4: Scenedesmus obliquus EN0004
v1.0, Scequ2611: Scenedesmus quadricauda LWG 002611, Celfi:Cellulomonas fimi .