Study sites and sampling
We sampled 13-28 brown anoles from each of 30 populations in
southeastern United States from Florida (N = 28 sites) and
Georgia (N = 2 sites; Table S1). Sites were organized along three
latitudinal transects (Figure S1), completed during three field trips in
March-July 2018. Sampling was limited to natural habitats with variable
understory light conditions and no evidence of artificial structures.
However, we sampled two populations (i.e., LOW and TIF; Table S1) from
landscaped vegetation around hotel grounds due to low lizard densities
in these areas. In all, we obtained 589 adult males, which were
transported to the animal care facility of Harvard University. We kept
the lizards for 5-9 weeks in the lab under uniform conditions in
individual enclosures (see De Meyer et al., 2019 for a detailed
enclosure description), during which time they were measured for a range
of related projects. Dewlap characteristics were scored for a subset of
570 lizards at 10-30 days after arrival from the field. All procedures
described here for housing and measuring lizards were approved by the
Harvard University Institutional Animal Care and Use Committee (IACUC
protocol # 26-11).